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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study

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Published on: August 16, 2019

Recent Developments Toward Antibody Engineering and Affinity Maturation.

Abhishek S Rathore¹, Animesh Sarker¹, Rinkoo D Gupta¹

¹Faculty of Life Sciences and Biotechnology, South Asian University, New Delhi-110021, India.

Protein and Peptide Letters

|September 27, 2018

Summary

This summary is machine-generated.

Recombinant antibody fragments offer a feasible alternative to full antibodies for therapeutics and diagnostics, enabling enhanced specificity and stability through protein engineering. Further advancements focus on affinity maturation and tailored pharmacokinetic properties for improved clinical applications.

Keywords:

Antibody fragment affinity maturation antibody engineering recombinant antibodies single chain variable fragment therapeutic antibody.

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Area of Science:

Biotechnology
Immunology
Protein Engineering

Background:

Monoclonal antibodies are crucial in healthcare for therapeutics and diagnostics.
Antibody fragments provide high antigen specificity and binding efficiency, with easier recombinant production.
Challenges remain in achieving high specificity and stability in antibody development.

Purpose of the Study:

To review the latest techniques in antibody fragment construction.
To highlight recent advancements in antibody affinity maturation.
To discuss the applications of recombinant antibodies.

Main Methods:

Protein engineering techniques for antibody fragment modification.
Construction of antibody fragments with fused Fc domains.
Affinity maturation strategies for enhanced antibody binding.

Main Results:

Antibody fragments retain binding efficiency and specificity of whole antibodies.
Recombinant production of antibody fragments is feasible in various host cells.
Protein engineering allows tailoring of antibody fragments for improved properties.

Conclusions:

Recent protein engineering advances offer options for modifying antibody fragments.
Tailoring antibody fragments improves stability, specificity, and pharmacokinetic profiles.
This review covers construction techniques, affinity maturation, and applications of recombinant antibodies.