Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pyrimidine dimers block simian virus 40 replication forks.

C A Berger, H J Edenberg

    Molecular and Cellular Biology
    |October 1, 1986
    PubMed
    Summary

    UV radiation creates DNA lesions that block replication forks in mammalian cells. This study used electron microscopy to show UV-induced pyrimidine dimers halt DNA replication fork progression, impacting DNA repair.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Parental but not child reports of affective lability in offspring predict conversion to bipolar disorder in youth at increased genetic risk.

    Journal of affective disorders·2026
    Same author

    Impact of heteroresistance on treatment outcomes of people with drug-resistant TB.

    IJTLD open·2024
    Same author

    Understanding patient-level costs of weekly isoniazid-rifapentine (3HP) among people living with HIV in Uganda.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2023
    Same author

    The association of polygenic risk for schizophrenia, bipolar disorder, and depression with neural connectivity in adolescents and young adults: examining developmental and sex differences.

    Translational psychiatry·2021
    Same author

    Willingness to accept reimbursement for visits to an HIV clinic for tuberculosis preventive therapy.

    The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease·2020
    Same author

    Genetic influences on eight psychiatric disorders based on family data of 4 408 646 full and half-siblings, and genetic data of 333 748 cases and controls - CORRIGENDUM.

    Psychological medicine·2018

    Area of Science:

    • Molecular Biology
    • Genetics
    • Cell Biology

    Background:

    • Ultraviolet (UV) light induces DNA lesions, primarily pyrimidine dimers.
    • These lesions impede DNA replication in mammalian cells, but the exact mechanism remains debated.
    • The controversy lies in whether replication forks stall temporarily or are completely blocked at lesions.

    Purpose of the Study:

    • To investigate the effect of UV-induced DNA lesions on replication fork progression.
    • To determine if UV lesions block replication fork movement or if forks bypass them.

    Main Methods:

    • Utilized electron microscopy to visualize replication forks in simian virus 40 (SV40) chromosomes.
    • Analyzed the location (symmetric vs. asymmetric) of replication forks relative to the origin in both UV-irradiated and control samples.

    Main Results:

    • A significant proportion of replication forks in UV-irradiated SV40 chromosomes were found in asymmetric locations.
    • This asymmetry indicates that UV lesions, specifically pyrimidine dimers, actively block replication fork progression.
    • The findings support the model where lesions impede the physical movement of the replication fork.

    Conclusions:

    • UV-induced pyrimidine dimers act as roadblocks, halting replication fork progression.
    • Leading strand synthesis is proposed as a key factor in fork blockage, as lesions in its template impede polymerization and thus fork movement.

    Related Experiment Videos