Corneal thickness, endothelial cell density, and morphological and morphometric features of corneal endothelial cells...

OBJECTIVE To determine corneal thickness (CT), endothelial cell density (ECD), and morphological and morphometric features of caprine eyes and to assess effects of aging on these variables. SAMPLE 27 healthy eyes of 19 Murciano-Granadina goats. PROCEDURES Goats were classified into 2 age groups (kids, 14 months old [14 eyes]; and adults, 7 to 10 years old [13 eyes]). The ECD and CT were calculated in the central cornea and 4 peripheral quadrants. Mean cell area (MCA), pleomorphism (percentage of hexagonal cells), and polymegathism were evaluated in the central cornea. RESULTS Median values for kids were determined for ECD (3,831 cells/mm²; inter-quartile [25th to 75th percentile] range [IQR], 3,669 to 4,011 cells/mm²), CT (608 μm; IQR, 573 to 655 μm), MCA (255 μm²; IQR, 243 to 272 μm²), pleomorphism (80.53%; IQR, 78.83% to 83.30%), and polymegathism (19; IQR, 18 to 22). Median values for adults were determined for ECD (2,101 cells/mm²; IQR, 1,966 to 2,251 cells/mm²), CT (706 μm; IQR, 670 to 730 μm), MCA (466 μm²; IQR, 425 to 507 μm²), pleomorphism (67.80%; IQR, 65.50% to 70.00%), and polymegathism (21; IQR, 15 to 26). Values differed significantly between the 2 groups for all variables, except polymegathism. For both groups, the dorsal and temporal quadrants were the thickest and thinnest, respectively. Ventral ECD was the lowest for both groups. CONCLUSIONS AND CLINICAL RELEVANCE ECD decreased with age, whereas MCA, pleomorphism, and CT increased. Moreover, differences among regions of the cornea indicated that the central cornea should not be considered as representative of the entire cornea.