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Colistin- and Carbapenem-Resistant Klebsiella pneumoniae Clinical_Isolates: Algeria

Hanane Yousfi1, Linda Hadjadj1, Iman Dandachi1

  • 11 Aix Marseille University , IRD, APHM, MEPHI, IHU-Mediterranean Infection, Marseille, France .

Microbial Drug Resistance (Larchmont, N.Y.)
|September 27, 2018
PubMed

Insights

This study examined Klebsiella pneumoniae ST101 resistance mechanisms. Researchers identified carbapenemase genes and colistin resistance mutations, revealing key factors in antibiotic resistance.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Klebsiella pneumoniae is a significant opportunistic pathogen.
  • Antimicrobial resistance in K. pneumoniae poses a global health threat.
  • Specific sequence types, like ST101, can exhibit unique resistance profiles.

Purpose of the Study:

  • To elucidate the molecular basis of colistin and carbapenem resistance in Klebsiella pneumoniae ST101.
  • To identify specific genetic determinants contributing to multidrug resistance in these strains.

Main Methods:

  • Whole-genome sequencing or PCR to identify resistance genes.
  • Analysis of mutations in genes associated with colistin resistance (e.g., pmrA/B, mgrB).
  • Antimicrobial susceptibility testing to confirm resistance phenotypes.

Main Results:

  • Identified blaCTX-M-15, blaTEM-183, and blaSHV-106 beta-lactamase genes in K. pneumoniae ST101.
  • Detected blaOXA-48 carbapenemase gene in two of the isolates.
  • Observed amino acid substitutions in PmrA/B and a truncated mgrB gene, linked to colistin resistance.

Conclusions:

  • Klebsiella pneumoniae ST101 strains harbor diverse carbapenemase and extended-spectrum beta-lactamase genes.
  • Colistin resistance is associated with specific mutations in the PmrA/B two-component system and mgrB gene.
  • Understanding these mechanisms is crucial for developing effective treatment strategies against resistant K. pneumoniae.
Keywords:
insertioncarbapenem resistancecolistin resistance

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