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Introduction to Innate and Adaptive Immunity01:21

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The human immune system is a complex defense mechanism that protects the body from harmful pathogens and foreign substances. It comprises two crucial components: innate and adaptive immunity.
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The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
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Self-Presentation01:25

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Self-presentation is a fundamental aspect of social interaction, shaping both how others perceive individuals and how they view themselves. This dynamic process influences behaviors in various social settings, often leading people to adjust their appearance, speech, and demeanor to align with their desired identity. While self-presentation can be deliberate or unconscious, it plays a critical role in interpersonal relationships and self-perception.Forms of Self-PresentationSelf-presentation can...
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People can go to great lengths to protect their self-image and present themselves in ways that they want others to see them. Sociologist Erving Goffman presented the idea that a person is like an actor on a stage. Calling his theory dramaturgy, Goffman believed that we use “impression management” to present ourselves to others as we hope to be perceived. Each situation is a new scene, and individuals perform different roles depending on who is present (Goffman, 1959). Think about...
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Related Experiment Video

Updated: Feb 4, 2026

Immunopeptidomics: Isolation of Mouse and Human MHC Class I- and II-Associated Peptides for Mass Spectrometry Analysis
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Innate Immune Signals Induce Anterograde Endosome Transport Promoting MHC Class I Cross-Presentation.

Mirjana Weimershaus1, François-Xavier Mauvais1, Loredana Saveanu1

  • 1INSERM, U1151, 75015 Paris, France; Université Paris Descartes, 75015 Paris, France; CNRS UMR8253, 75015 Paris, France.

Cell Reports
|September 27, 2018
PubMed
Summary
This summary is machine-generated.

Toll-like receptor 4 (TLR4) signaling regulates early endosome trafficking via Rab14 and KIF16b, delaying phagosome maturation. This mechanism is crucial for antigen cross-presentation by dendritic cells, impacting T-cell immunity and immune tolerance.

Keywords:
MHC class IRab14antigen presentationcross-presentationdendritic cellendosomekinesinsmall GTPase

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Area of Science:

  • Immunology
  • Cell Biology
  • Microbiology

Background:

  • Cross-presentation of antigens by dendritic cells is vital for T-cell immunity and immune tolerance.
  • Pathogen survival within intracellular vacuoles is linked to delayed vesicle maturation.
  • Mechanisms maintaining early endosome/phagosome identity remain unclear.

Purpose of the Study:

  • To elucidate the role of early endosome trafficking in antigen cross-presentation.
  • To investigate the involvement of Rab14 and KIF16b in regulating phagosome maturation.
  • To understand how innate immune signals influence dendritic cell function.

Main Methods:

  • Investigated the interaction between Rab14 and KIF16b upon Toll-like receptor 4 (TLR4) and Fc receptor ligation.
  • Utilized overexpression and knockdown strategies for Rab14 and KIF16b.
  • Assessed the impact of altered microtubule transport on phagosome maturation and pathogen killing.
  • Quantified changes in cross-presentation efficiency under various experimental conditions.

Main Results:

  • TLR4 and Fc receptor ligation promotes Rab14-KIF16b interaction, mediating anterograde microtubule transport of endosomes.
  • Rab14 recruitment to phagosomes delays their maturation and pathogen killing.
  • Enhanced anterograde transport (via Rab14 overexpression or GTP-bound state) or inhibited retrograde transport upregulates cross-presentation.
  • Reduced Rab14 expression, destabilized Rab14 endosomes, or inhibited KIF16b-mediated transport compromises cross-presentation.

Conclusions:

  • Innate immune signaling regulates early endosome trafficking through the Rab14-KIF16b pathway.
  • This trafficking control is a critical determinant of antigen cross-presentation efficiency by dendritic cells.
  • The findings provide insights into the interplay between innate immunity, endosomal trafficking, and adaptive immune responses.