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Tunable Hydrogels from Pulmonary Extracellular Matrix for 3D Cell Culture
Published on: January 17, 2017
Porcine Breast Extracellular Matrix Hydrogel for Spatial Tissue Culture
Girdhari Rijal1, Jing Wang2, Ilhan Yu3
1Department of Biomedical Sciences, Elson S. Floyd College of Medicine, Washington State University, Spokane, WA 99202, USA. girdhari.rijal@wsu.edu.
Abstract:
Porcine mammary fatty tissues represent an abundant source of natural biomaterial for generation of breast-specific extracellular matrix (ECM). Here we report the extraction of total ECM proteins from pig breast fatty tissues, the fabrication of hydrogel and porous scaffolds from the extracted ECM proteins, the structural properties of the scaffolds (tissue matrix scaffold, TMS), and the applications of the hydrogel in human mammary epithelial cell spatial cultures for cell surface receptor expression, metabolomics characterization, acini formation, proliferation, migration between different scaffolding compartments, and in vivo tumor formation. This model system provides an additional option for studying human breast diseases such as breast cancer.
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