Micro-computed tomography and histology to explore internal morphology in decapod larvae

Area of Science:

• Marine Biology
• Developmental Biology
• Zoology

Background:

• Internal morphology of crustacean larvae traditionally studied via destructive methods.
• Limitations exist with dissection and microscopy alone.
• Advancements in imaging offer new possibilities for non-destructive analysis.

Purpose of the Study:

• To combine micro-computed tomography (micro-CT) and histology for studying decapod larval internal morphology.
• To use the common spider crab (Maja brachydactyla) as a model organism.
• To overcome the individual limitations of micro-CT and histology.

Main Methods:

• Utilized micro-computed tomography (micro-CT) for gross morphological analysis.
• Employed histology for detailed tissue and cellular identification.
• Integrated both techniques to leverage synergistic benefits.

Main Results:

• Successfully identified organs, revealing gross morphology and histological organization.
• Micro-CT highlighted exoskeleton, musculature, digestive, and nervous systems.
• Histology confirmed organ identity and identified cell types, resolving discrepancies in nervous system literature.

Conclusions:

• The combined micro-CT and histology approach provides superior results compared to individual methods.
• This integrated technique offers comprehensive insights into decapod larval internal morphology.
• Significant developmental changes during metamorphosis to the megalopa stage were detailed.