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Updated: Feb 4, 2026

Near Simultaneous Laser Scanning Confocal and Atomic Force Microscopy Conpokal on Live Cells
Published on: August 11, 2020
Cellular in vivo 3D imaging of the cornea by confocal laser scanning microscopy
Sebastian Bohn1, Karsten Sperlich1, Stephan Allgeier2
1Department of Ophthalmology, University Medical Center Rostock, 18057 Rostock, Germany.
Abstract:
We present an in vivo confocal laser scanning microscopy based method for large 3D reconstruction of the cornea on a cellular level with cropped volume sizes up to 266 x 286 x 396 µm3. The microscope objective used is equipped with a piezo actuator for automated, fast and precise closed-loop focal plane control. Furthermore, we present a novel concave surface contact cap, which significantly reduces eye movements by up to 87%, hence increasing the overlapping image area of the whole stack. This increases the cuboid volume of the generated 3D reconstruction significantly. The possibility to generate oblique sections using isotropic volume stacks opens the window to slit lamp microscopy on a cellular level.
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