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Updated: Feb 4, 2026

Micro-particle Image Velocimetry for Velocity Profile Measurements of Micro Blood Flows
Published on: April 25, 2013
Double-pulse laser illumination method for measuring fast cerebral blood flow velocities in the deep brain using a
Minkyung Kim1,2, Jinki Hong1,2, Hyun-Joon Shin1,2
1Center for Bionics, Biomedical Research Institute, Korea Institute of Science and Technology, Seoul 02792, South Korea.
Abstract:
We present a new fiber-bundle-based endomicroscopy system to measure the fast cerebral blood flow (CBF) velocity in blood vessels located between the surface and the deep brain of living animals. The CBF velocity is obtained by measuring the displacement of the partially overlapped red blood cell images directly, using double-pulse 532-nm laser illumination. The proposed method could measure CBF in blood vessels with diameters ranging from 4 μm to 42 μm and could measure CBF velocities up to 3.2 μm/ms for different vessel diameters at a depth of 2.1 mm from the brain surface in a living mouse.
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