Diagnosis of dermatophytosis using single fungus endogenous fluorescence spectrometry

We propose to use a single fungus endogenous fluorescence spectrometry base on a hyperspectral fluorescence microscope for the diagnosis of dermatophytosis. Dermatophyte samples, including Aspergillus, Trichophyton rubrum, Microsporum gypseum, and Microsporum canis were imaged, and the endogenous fluorescence spectrum of a single fungus was calculated. High contrast fluorescence images and endogenous fluorescence spectrum of the single fungus were used to identify the type of dermatophyte. Morphologically similar Microsporum gypseum and Microsporum canis can be distinguished using an endogenous fluorescence spectrum of the single fungus. Meanwhile, our result showed that the sensitivity and specificity of identifying Microsporum gypseum were 95% and 93%, and the sensitivity and specificity of identifying Microsporum canis were 94% and 93%.