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Applications of the Single-probe: Mass Spectrometry Imaging and Single Cell Analysis under Ambient Conditions
Published on: June 14, 2016
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Application of Various Normalization Methods for Microscale Analysis of Tissues Using Direct Analyte Probed
Janella de Jesus1,2, Josephine Bunch2, Guido Verbeck3
1Department of Chemistry , University of Surrey , Guildford , Surrey GU2 7XH , United Kingdom.
Analytical Chemistry
|September 28, 2018
Summary
Direct analyte probed nanoextraction (DAPNe) improves mass spectrometry sensitivity. This study enhances DAPNe
Area of Science:
- Analytical Chemistry
- Mass Spectrometry
- Nanotechnology
Background:
- Direct analyte probed nanoextraction (DAPNe) offers high sensitivity for microscale sample analysis.
- Previous applications include single-cell and organelle analysis.
- Quantitative capabilities of DAPNe require further evaluation.
Purpose of the Study:
- To investigate normalization techniques for improving DAPNe quantitative accuracy.
- To assess methods for internal standard incorporation.
- To explore normalization strategies when internal standards are unavailable.
Main Methods:
- Investigated two internal standard incorporation methods on test substrates mimicking biological matrices.
- Evaluated normalization to extraction spot area.
- Assessed normalization to matrix compounds.
Main Results:
- Sprayed internal standards significantly reduced observed variability.
- Normalization to the extracted area also reduced variability in certain scenarios.
- The study identified effective strategies for enhancing DAPNe quantification.
Conclusions:
- Normalization techniques are crucial for improving the quantitative performance of DAPNe.
- Internal standards, particularly sprayed ones, are effective for reducing variability.
- Matrix-based normalization offers an alternative when internal standards are not feasible.
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