Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Quality Control01:05

Quality Control

2.6K
Quality control is one of the three cyclical quality assurance activities that help keep a system under statistical control. Typical quality control activities include creating quality control charts, conducting proficiency testing, and documenting and archiving results.
Quality control helps track data, visualize trends, and identify variations, making it easier to detect deviations that may affect the accuracy of an analysis. One way to do this is by generating a quality control chart, which...
2.6K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

6.0K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.0K
Neural Control of Respiration01:18

Neural Control of Respiration

4.9K
The neural regulation of respiration is a meticulously coordinated process primarily controlled by the respiratory centers located within the brainstem. These centers, composed of specialized neurons, transmit nerve impulses that control the contraction and relaxation of our respiratory muscles.
Respiratory Centers in the Brainstem
Two primary areas comprise the respiratory center: the medullary respiratory center in the medulla oblongata and the pontine respiratory group in the pons. The...
4.9K
Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.8K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.8K
Zygotic Development And Stem Cell Formation01:10

Zygotic Development And Stem Cell Formation

6.8K
The development of all multicellular organisms starts with the fusion of haploid cells called sperm and egg to form a diploid zygote. A zygote is a totipotent cell that can develop into a complete organism. The zygote undergoes cell division or cleavage to form an 8-cell mass. Until this stage, the cells are spherical, loosely attached, and remain totipotent. Totipotent cells are capable of developing both the embryonic and the extraembryonic tissues. However, as they continue to divide, they...
6.8K
Adult Stem Cells01:33

Adult Stem Cells

33.9K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary soy shapes the microbiome to induce commensal-specific T follicular helper cells and IgA production.

Immunity·2026
Same author

Germ granule components regulate endosomal switching during the oocyte-to-embryo transition in Caenorhabditis elegans.

Communications biology·2026
Same author

Transmembrane ROOM proteins ensure rooms for germ cells by maintaining intercellular bridges.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Biallelic variants in FAT3 cause axonal neuropathy with multisystem neurodevelopmental features.

Genetics in medicine : official journal of the American College of Medical Genetics·2026
Same author

Mitochondrial control of glycerolipid synthesis by a PEP shuttle.

Cell·2026
Same author

Electrolyzed Hydrogen Water Improves Chemosensitivity to Anticancer Drugs by Potently Suppressing Autophagy.

Journal of cellular and molecular medicine·2026

Related Experiment Video

Updated: Feb 4, 2026

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids
09:18

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Published on: September 19, 2017

11.0K

Rer1-mediated quality control system is required for neural stem cell maintenance during cerebral cortex development.

Taichi Hara1,2, Ikuko Maejima1, Tomoko Akuzawa1

  • 1Laboratory of Molecular Traffic, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Gunma, Japan.

Plos Genetics
|September 28, 2018
PubMed
Summary

Retrieval receptor Rer1 is crucial for maintaining sufficient cell surface expression of the gamma-secretase complex. This ensures proper Notch signaling and neural stem cell maintenance during mouse cerebral cortex development.

More Related Videos

Enumeration of Neural Stem Cells Using Clonal Assays
10:32

Enumeration of Neural Stem Cells Using Clonal Assays

Published on: October 4, 2016

8.8K
Author Spotlight: Simple and Efficient Neural Retina Organoid Production for Disease Modeling
05:03

Author Spotlight: Simple and Efficient Neural Retina Organoid Production for Disease Modeling

Published on: December 22, 2023

1.9K

Related Experiment Videos

Last Updated: Feb 4, 2026

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids
09:18

Modelling Zika Virus Infection of the Developing Human Brain In Vitro Using Stem Cell Derived Cerebral Organoids

Published on: September 19, 2017

11.0K
Enumeration of Neural Stem Cells Using Clonal Assays
10:32

Enumeration of Neural Stem Cells Using Clonal Assays

Published on: October 4, 2016

8.8K
Author Spotlight: Simple and Efficient Neural Retina Organoid Production for Disease Modeling
05:03

Author Spotlight: Simple and Efficient Neural Retina Organoid Production for Disease Modeling

Published on: December 22, 2023

1.9K

Area of Science:

  • Cell biology
  • Developmental biology
  • Neuroscience

Background:

  • Rer1 acts as a retrieval receptor for endoplasmic reticulum (ER) proteins.
  • The physiological roles of Rer1 in mammalian development are not well understood.
  • The gamma-secretase complex is vital for modulating Notch signaling.

Purpose of the Study:

  • To investigate the role of the Rer1-mediated quality control system in mammalian development.
  • To determine the impact of Rer1 on gamma-secretase complex expression and Notch signaling in the developing brain.

Main Methods:

  • Depletion of Rer1 in the mouse cerebral cortex.
  • Assessment of neural stem cell numbers and cerebral cortex morphology.
  • Measurement of gamma-secretase activity and Notch signaling pathway components.
  • Analysis of gamma-secretase complex localization and degradation in Rer1-deficient cells.

Main Results:

  • Rer1 depletion led to decreased neural stem cell numbers and cerebral cortex malformations.
  • Loss of Rer1 significantly reduced gamma-secretase activity and downregulated Notch signaling.
  • In Rer1-deficient cells, gamma-secretase components were aberrantly trafficked to lysosomes for degradation.
  • Cell surface expression of the gamma-secretase complex was significantly reduced in the absence of Rer1.

Conclusions:

  • Rer1 is essential for maintaining adequate cell surface expression of the gamma-secretase complex.
  • Rer1 plays a critical role in regulating Notch signaling and neural stem cell maintenance during cerebral cortex development.
  • The Rer1-mediated quality control system is vital for proper mammalian brain development.