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Nanosensors to Detect Protease Activity In Vivo for Noninvasive Diagnostics
Published on: July 16, 2018
A novel AuNPs-based nanosensors for smart detection of NO with low concentration
Yumei Peng1, Lixia Qin1, Shi-Zhao Kang1
1School of Chemical and Environmental Engineering, Center of Graphene Research, Shanghai Institute of Technology, 100 Haiquan Road, Shanghai 201418, China.
Abstract:
A novel method for detection of nitric oxide (NO) was developed by the structural transition of oxidized cytochrome c (Cyt c III) on gold nanoparticles (AuNPs)/glycine (Gly)/graphene quantum dots (GQDs) composite using surface-enhanced Raman scattering (SERS) spectroscopy. Importantly, the composite can be used as a good SERS substrate for the selective detection of trace NO based on the interaction of the Cyt c (III) and NO, and the structural transition of oxidized Cyt c III on AuNPs/Gly/GQDs composite. The NO concentration in real seawater sample was determined to be 7.28 × 10-8 M. Combined with the interaction of the Cyt c (III) and NO, and the structural transition of oxidized Cyt c (III) on AuNPs/Gly/GQDs composite, a novel AuNPs-based SERS probe for the selective detection of trace NO was constructed, and the detection limit for NO can reach about ~10-8 M.
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