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Toxic Dose prediction of Chemical Compounds to Biomarkers using an ANOVA based Gene Expression Analysis

Mohammad Nazmol Hasan1,2, Zobaer Akond1,3, Md Jahangir Alam1

  • 1Bioinformatics Lab, Department of Statistics, University of Rajshahi, Rajshahi-6205, Bangladesh.

Bioinformation
|September 29, 2018
PubMed

Insights

This study introduces a new ANOVA model to identify toxic chemical compound (CC) doses and their biomarker genes. This method improves efficiency in drug discovery and toxicogenomic research.

Area of Science:

  • Toxicogenomics
  • Computational Biology
  • Pharmacology

Background:

  • Toxicogenomic studies are vital for optimizing chemical compound (CC) toxicity levels and identifying regulated biomarker genes, crucial for drug discovery.
  • Existing tools like ToxDB and Toxygates use t-tests but are insufficient for determining the specific CC doses that regulate biomarker genes.

Purpose of the Study:

  • To develop and present an improved computational model for identifying toxicogenomic biomarker genes and their corresponding CC regulatory doses.
  • To enhance the efficiency of toxicogenomic biomarker discovery in drug development and chemical safety assessment.

Main Methods:

  • A novel approach utilizing a one-way ANOVA model combined with Tukey's HSD test was developed.
  • This method was applied to analyze glutathione metabolism pathway data to identify significant CC doses and regulated genes.

Main Results:

  • The analysis identified specific toxic CC doses: high and middle doses for acetaminophen and nitrofurazone, and a high dose for methapyrilene.
  • Seven key toxicogenomic biomarker genes were identified: Gstp1, Gsr, Mgst2, Gclm, G6pd, Gsta5, and Gclc.

Conclusions:

  • The proposed ANOVA and Tukey's HSD test model offers improved efficiency in identifying toxic CC doses and associated biomarker genes.
  • This method provides a more precise tool for toxicogenomic analysis, supporting safer drug development and chemical risk assessment.
Keywords:
DoseOne-way ANOVATukey's HSD testchemical Compoundsgene expressiontoxicogenomic biomarker

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