Hydrolysis of cassava starch by co-immobilized multi-microorganisms of Loog-Pang (Thai rice cake starter) for ethanol
Arnon Khamkeaw1, Muenduen Phisalaphong1
1Chemical Engineering Research Unit for Value Adding of Bioresources, Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330 Thailand.
Abstract:
Loog-Pang (Thai rice cake starter) is an effective and inexpensive microbial source for the hydrolysis of cassava starch to glucose. A process for hydrolysis of cassava starch to glucose by Loog- Pang was improved by co-immobilized multi-microorganisms (IC) using thin shell silk cocoon (TSC). After incubation at 35°C for 120 h, the IC-TSC system converted 20% w/v cassava starch slurry into clear glucose syrup containing a glucose concentration of 145.5 g/L (composed of 98.8% glucose and 1.2% oligosaccharides), with little or no contamination by microorganisms. The glucose concentration from the starch hydrolysis process using the IC-TSC system was approximately 1.3 times more than that of suspended cultures (SC). The starch hydrolysate could be used as the carbon source for ethanol fermentation without sterilization. A concentration of ethanol of 71.2 g/L (9.1%, v/v) was obtained at 36 h fermentation of the starch hydrolysate by Saccharomyces cerevisiae M30.
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