Related Experiment Video
Updated: Feb 4, 2026

The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Characterization of macrophage-activating lactic acid bacteria isolated from Mukeunji
Jae-Hwan Kim1, Jingmei Li1, Seon-Kyeong Han1
1Institute of Life Sciences & Resources and Department of Food Sciences & Biotechnology, Kyung Hee University, Yongin, Gyeonggi, 17104 Korea.
Abstract:
Lactic acid bacteria (LAB) were reported to comprise the majority of the bacterial population in mukeunji, a long-fermented kimchi. This current study investigated the probiotic abilities of LAB isolated from mukeunji. Forty bacterial strains from mukeunji were identified by SDS-PAGE gel patterns, which were further tested for acid and bile tolerance. An assessment of the immuneboosting effects of these strains in RAW264.7 cells was also carried out by measurement of secreted proinflammatory cytokines. Heat-killed LAB were also tested for their contribution to cytokine production because certain LAB are not durable in the digestive tract. Immune-boosting strains were further characterized by SDS-PAGE of whole-cell protein and 16S rRNA gene sequencing, resulting in the identification of Lactobacillus plantarum, L. sakei, Weissella cibaria, and Pediococcus parvulus. These data and the fact that mukeunji is highly consumed in Korea, together, highlight the need of detailed epidemiological and animal feeding studies.
More Related Videos
10:23Characterizing Mediated Extracellular Electron Transfer in Lactic Acid Bacteria with a Three-Electrode, Two-Chamber Bioelectrochemical System
Published on: August 23, 2024
07:46Isolation, Characterization, and Purification of Macrophages from Tissues Affected by Obesity-related Inflammation
Published on: April 3, 2017
Related Concept Videos
Acid–Base Equilibria: Activity-Based Definition of pH
In solutions of very low ionic strength—for example, pure water—the...
Prokaryotic Transcriptional Activators and Repressors
Transcription of prokaryotic...
The Roles of Bacteria and Fungi in Plant Nutrition
Amino acids
Polyprotic Acids
Mixtures of Acids
A Mixture of a Strong Acid and a Weak Acid
In a mixture of a strong acid and a weak acid, the strong acid dissociates completely and becomes a source of almost all the hydronium ions...