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Classification and fatty acid composition analysis of Cronobacter spp. isolated from powdered infant formula in China

Xiaojuan Yang1, Qingping Wu1, Jumei Zhang1

  • 1Guangdong Institute of Microbiology, State Key Laboratory of Applied Microbiology Southern China, Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application, Guangdong Open Laboratory of Applied Microbiology, Guangzhou, 510070 China.

Food Science and Biotechnology
|September 29, 2018
PubMed

Insights

Cronobacter sakazakii and Cronobacter malonaticus are common in Chinese powdered infant formula. Cellular fatty acid analysis effectively differentiates these Cronobacter species, aiding in epidemiological studies.

Area of Science:

  • Microbiology
  • Food Safety
  • Genetics

Background:

  • Enterobacter sakazakii (E. sakazakii) is a pathogen found in powdered infant formula (PIF). Accurate identification and differentiation of Cronobacter species are crucial for public health.
  • Previous identification of E. sakazakii strains from PIF necessitates further species-level classification and diversity analysis.

Purpose of the Study:

  • To classify E. sakazakii strains from PIF to the species level using recN gene sequencing and biochemical tests.
  • To determine the distribution of Cronobacter species in China.
  • To investigate strain diversity using cellular fatty acid (CFA) analysis.

Main Methods:

  • recN gene sequencing for species identification.
  • Biochemical testing for phenotypic characterization.
  • Cellular Fatty Acid (CFA) analysis for strain diversity and differentiation.

Main Results:

  • Of 24 E. sakazakii isolates, 23 were identified as Cronobacter sakazakii and one as Cronobacter malonaticus.
  • All 23 C. sakazakii isolates displayed identical CFA profiles.
  • The C. malonaticus isolate was distinguished from C. sakazakii by significant differences in C12:0, C14:0, and C17:0 fatty acid content.

Conclusions:

  • Cronobacter sakazakii and C. malonaticus are prevalent Cronobacter species in PIF in China.
  • Distinct CFA profiles differentiate C. sakazakii and C. malonaticus isolates.
  • CFA analysis offers a valuable alternative method for identifying and differentiating Cronobacter species in epidemiological investigations.

Keywords:
Cronobactercellular fatty acidsclassificationpowdered infant formula

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