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Role of phage-antibiotic combination in reducing antibiotic resistance in Staphylococcus aureus

Ara Jo1, Jeongjin Kim1, Tian Ding2

  • 11Department of Medical Biomaterials Engineering and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon, 24341 Korea.

Food Science and Biotechnology
|September 29, 2018
PubMed

Insights

Phage and antibiotic combination therapy effectively reduces Staphylococcus aureus growth and biofilm formation. This synergistic approach prevents the development of antibiotic and phage resistance in bacteria.

Area of Science:

  • Microbiology
  • Bacteriology
  • Antimicrobial Resistance

Background:

  • Antibiotic resistance poses a significant threat to public health.
  • Staphylococcus aureus is a common pathogen associated with various infections.
  • Novel strategies are needed to combat antibiotic-resistant bacteria.

Purpose of the Study:

  • To evaluate the synergistic effect of phage and antibiotic combination therapy against Staphylococcus aureus.
  • To assess the impact of this combination on bacterial growth, biofilm formation, and resistance development.

Main Methods:

  • Treatment of Staphylococcus aureus with ciprofloxacin, phages, or a combination thereof.
  • Quantification of bacterial reduction over time.
  • Assessment of biofilm formation.
  • Determination of mutant frequencies for antibiotic and phage resistance.

Main Results:

  • Combination therapy demonstrated the most effective inhibition of S. aureus growth, achieving over 4 log reduction in 18 hours.
  • Significant reduction in biofilm formation was observed with combination treatment.
  • While ciprofloxacin alone induced resistance to both antibiotic and phage, the combination therapy prevented the emergence of resistant strains.

Conclusions:

  • Phage-antibiotic synergy is a promising strategy for controlling Staphylococcus aureus infections.
  • Combination therapy effectively reduces bacterial load and biofilm formation.
  • This approach mitigates the risk of developing antibiotic and phage resistance, offering a potential solution for clinical applications.
Keywords:
Staphylococcus aureusantibiotic resistanceciprofloxacinmutant frequencyphage-antibiotic combination

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