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Updated: Feb 4, 2026

Phenotypic Analysis and Isolation of Murine Hematopoietic Stem Cells and Lineage-committed Progenitors
Published on: July 8, 2012
What do the lineage tracing studies tell us? Consideration for hematopoietic stem cell origin, dynamics, and
Nathalia Azevedo Portilho1, Michihiro Kobayashi1, Momoko Yoshimoto2
1Center for Stem Cell and Regenerative Medicine at Institute of Molecular Medicine, McGovern Medical School, University of Texas Health Science Center at Houston, 1825 Pressler Street, Houston, 77030, USA.
Abstract:
The recent advance of technologies enables us to trace the cell fate in vivo by marking the cells that express the gene of interest or by barcoding them at a single cell level. Various tamoxifen-inducible Cre-recombinase mice combined with Rosa-floxed lines are utilized. In this review, with the results revealed by lineage tracing assays, we re-visit the long-standing debate for the origin of hematopoietic stem cells in the mouse embryo, and introduce the view of native hematopoiesis, and possible leukemic-initiating cells emerged during fetal stages.
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