Related Experiment Video For Hedgehog signaling
Updated: Feb 4, 2026

Preparation of Exosomes for siRNA Delivery to Cancer Cells
Published on: December 5, 2018
Upstream Hedgehog signaling components are exported in exosomes of cervical cancer cell lines
Anjali Bhat1, Aman Sharma2, Alok Chandra Bharti1
1Molecular Oncology Laboratory, Department of Zoology, North Campus, University Of Delhi, New Delhi 110007, India.
Aim:
To investigate export of Hedgehog pathway (Hh) proteins Patched1, Smoothened, Sonic hedgehog and Indian hedgehog in cervical cancer cell line (CaCx) exosomes.
Methods:
Exosomes were isolated and characterized by Western blotting, scanning electron microscopy and in a colorimetric assay. Nucleic acids (RNA, DNA) and protein content of exosomes were analyzed. Hh pathway proteins in exosomes were detected using Western blotting.
Results:
CaCx secrete bio-macromolecule (DNA, RNA and proteins) enriched exosomes. CaCx exosomes contained higher amount of RNA with respect to DNA. CaCx preferentially exported Hh proteins (Patched1, Smoothened, Sonic hedgehog, Indian hedgehog) in their exosomes. Cellular uptake assay revealed rapid internalization of CaCx exosomes in human umbilical vein endothelial cells.
Conclusion:
Our study showed that Hh proteins are exported in CaCx exosomes.
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