Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

25.2K
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
25.2K
Pre-mRNA Processing: RNA Splicing01:36

Pre-mRNA Processing: RNA Splicing

6.9K
6.9K
RNA Editing02:23

RNA Editing

9.9K
RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
9.9K
RNA Splicing01:32

RNA Splicing

60.6K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.6K
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

11.9K
The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
11.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Integrated Transcriptome Landscape of mRNAs, lncRNAs, circRNAs, and miRNAs Reveals Molecular Regulatory Networks of Sex Differentiation in the Zig-Zag Eel (<i>Mastacembelus armatus</i>).

International journal of molecular sciences·2026
Same author

Restoring BECN1-mediated autophagy mitigates acute lung injury caused by zinc oxide nanoparticles.

Free radical biology & medicine·2026
Same author

Multiplexed, precise genome engineering in monocots with twin prime editing systems.

Nature biotechnology·2026
Same author

Perioperative pulmonary infection prevention and management in adult patients after coronary artery bypass grafting: a summary of best evidence.

BMC cardiovascular disorders·2026
Same author

LOX-A4 shapes Triticum urartu gene pools and contributions to the A subgenome of polyploid wheat.

Nature communications·2026
Same author

Fecal microbiota transplantation for intestinal rehabilitation after GI bleeding and perforation post-cardiac transplant: a case report.

Frontiers in medicine·2026

Related Experiment Video

Updated: Feb 4, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.5K

Manipulating mRNA splicing by base editing in plants.

Chenxiao Xue1,2, Huawei Zhang1, Qiupeng Lin1,2

  • 1State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China.

Science China. Life Sciences
|September 30, 2018
PubMed
Summary
This summary is machine-generated.

Scientists developed a new base editing tool to precisely control gene splicing in plants. This method enables the study of alternative splicing functions, revealing new insights into plant responses to hormones and DNA damage.

Keywords:
alternative 5′ splicing sitebase editingintron retentionsplicing

More Related Videos

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.6K

Related Experiment Videos

Last Updated: Feb 4, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

5.5K
Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells
10:06

Engineering Artificial Factors to Specifically Manipulate Alternative Splicing in Human Cells

Published on: April 26, 2017

9.4K
mRNA Interactome Capture from Plant Protoplasts
12:29

mRNA Interactome Capture from Plant Protoplasts

Published on: July 28, 2017

9.6K

Area of Science:

  • Molecular Biology
  • Plant Science
  • Genetics

Background:

  • Gene expression is regulated by constitutive and alternative splicing of precursor-mRNAs (pre-mRNAs).
  • Alternative splicing (AS) significantly expands proteomic diversity and gene regulatory functions.
  • Studying specific spliced isoforms in plants is challenging due to the lack of suitable mutants.

Purpose of the Study:

  • To develop an efficient tool for manipulating plant gene splicing.
  • To investigate the functional roles of specific splicing events in plants.

Main Methods:

  • Utilized a Cas9-directed base editor to convert 5' splice sites (GT to AT) in four Arabidopsis genes.
  • Applied base editing to silence alternative splicing of HAB1.1 and perturb alternative splicing of RS31A.
  • Modified constitutive splicing of Act2 to analyze intron-mediated enhancement (IME).

Main Results:

  • Silencing alternative splicing of HAB1.1 confirmed its role in abscisic acid signaling.
  • Perturbing alternative splicing of RS31A identified its function in response to genotoxic stress.
  • Altering constitutive splicing of Act2 facilitated the analysis of intron-mediated enhancement.

Conclusions:

  • A novel base editing strategy enables efficient manipulation of gene splicing in plants.
  • This tool facilitates functional studies of splicing regulation and specific mRNA isoforms.
  • The approach is applicable for investigating gene splicing in plants and other eukaryotes.