A guide to microRNA-mediated gene silencing
Related Experiment Video
Updated: Feb 4, 2026

Agrobacterium-Mediated Virus-Induced Gene Silencing Assay In Cotton
Published on: August 20, 2011
Miguel Quévillon Huberdeau1,2, Martin J Simard1,2
1Oncology division (St-Patrick Research Group in Basic Oncology), CHU de Québec-Université Laval Research Center, Quebec City, Canada.
Abstract:
As an important regulator of gene expression in eukaryotes, microRNAs (miRNAs) have been subject to extensive research to understand their pleiotropic roles in development and pathological processes. Here, we review strategies that have been developed to map microRNA-target interactions and the most effective methods to attribute post-transcriptional gene regulation through miRNA-mediated silencing. We further discuss the importance of the biological context for silencing.
Related Concept Videos
MicroRNAs
MicroRNAs
Organization of Genes
Receptor-mediated Endocytosis
Gene Flow
Gene Conversion

