Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Additional Subnuclear Structures02:10

Additional Subnuclear Structures

5.4K
The eukaryotic nucleus is a double membrane-bound organelle that contains nearly all of the cell’s genetic material in the form of chromosomes. It is rightly called the “brain” of the cell as it shoulders the responsibility of responding to various physiological processes, stress, altered metabolic conditions, and other cellular signals. 
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles,...
5.4K
Conjugate Addition (1,4-Addition) vs Direct Addition (1,2-Addition)01:27

Conjugate Addition (1,4-Addition) vs Direct Addition (1,2-Addition)

4.3K
α,β-Unsaturated carbonyl compounds with two electrophilic sites, the carbonyl carbon, and the β carbon, are susceptible to nucleophilic attack via two modes: conjugate or 1,4-addition and direct or 1,2-addition.
Conjugate addition results in a thermodynamically stable product. The reaction retains the stronger C=O bond at the expense of the weaker C=C π bond. The process is slow as the β carbon is less electrophilic than the carbonyl carbon.
Direct addition products are...
4.3K
Protein Complex Assembly02:41

Protein Complex Assembly

16.8K
Proteins can form homomeric complexes with another unit of the same protein or heteromeric complexes with different types.  Most protein complexes self-assemble spontaneously via ordered pathways, while some proteins need assembly factors that guide their proper assembly. Despite the crowded intracellular environment, proteins usually interact with their correct partners and form functional complexes.
Many viruses self-assemble into a fully functional unit using the infected host cell to...
16.8K
Conjugate Addition of Enolates: Michael Addition01:08

Conjugate Addition of Enolates: Michael Addition

3.6K
The attack of a nucleophile at the β carbon of an α,β-unsaturated carbonyl compound is called conjugate addition. Conjugate addition reactions of active methylene compounds, such as β-diketones, β-keto esters, β-keto nitriles, and α-nitro ketones, are called Michael addition reactions.
3.6K
Components of Stress01:23

Components of Stress

542
Stress analysis under multiple loading conditions is intricate, necessitating a comprehensive grasp of normal and shearing stresses. Consider a small cube at point O, subjected to stress on all six faces, visible or not. Normal stress components σx, σy, σz act perpendicularly to the x, y, and z axes. Shearing stress components τxy and τxz are exerted on faces perpendicular to these axes.
Interestingly, the hidden cube faces also experience these stresses, equal and...
542
Components of Language01:24

Components of Language

821
Language, whether spoken, signed, or written, consists of specific components: lexicon and grammar. The lexicon is the vocabulary of a language, comprising its words. Grammar is the set of rules used to convey meaning through the lexicon. For example, English grammar adds “-ed” to most verbs to indicate past tense. Words are formed by combining phonemes, which are the basic sound units of a language. Different languages have different sets of phonemes (e.g., “ah” vs.
821

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Insights on the spatial distribution of the <i>Pseudomonas aeruginosa</i> secondary metabolites under swarming motility-inducing conditions using mass spectrometry imaging.

Microbiology spectrum·2025
Same author

D-Amino acids affect Pseudomonas aeruginosa biofilm and quorum sensing molecules in lung infection models developed under a cystic fibrosis environment.

Scientific reports·2025
Same author

Use of analytical strategies to understand spatial chemical variation in bacterial surface communities.

Journal of bacteriology·2025
Same author

<i>Pseudomonas aeruginosa</i> alkyl quinolone response is dampened by <i>Enterococcus faecalis</i>.

mBio·2024
Same author

<i>Pseudomonas aeruginosa</i> Alkyl Quinolone Response is dampened by <i>Enterococcus faecalis</i>.

bioRxiv : the preprint server for biology·2024
Same author

Alkyl quinolones mediate heterogeneous colony biofilm architecture that improves community-level survival.

Journal of bacteriology·2024

Related Experiment Video

Updated: Feb 4, 2026

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.8K

Testing the component additivity approach to surface complexation modeling using a novel cadmium-specific fluorescent

Clayton R Johnson1, Juliane Hopf1, Joshua D Shrout2

  • 1Department of Civil & Environmental Engineering & Earth Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.

Journal of Colloid and Interface Science
|September 30, 2018
PubMed
Summary

This study validates the component additivity (CA) approach for predicting cadmium (Cd) adsorption in complex systems. A novel fluorescent probe technique accurately quantifies Cd distribution, supporting CA model reliability for environmental applications.

Keywords:
AdsorptionBacteriaCadmiumConfocal laser scanning microscopyFluorescent probeLeadmium GreenSurface complexation modeling

More Related Videos

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures
09:12

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures

Published on: August 10, 2017

8.0K
The Measurement of Unsteady Surface Pressure Using a Remote Microphone Probe
08:53

The Measurement of Unsteady Surface Pressure Using a Remote Microphone Probe

Published on: December 3, 2016

7.4K

Related Experiment Videos

Last Updated: Feb 4, 2026

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium
09:33

An Anaerobic Biosensor Assay for the Detection of Mercury and Cadmium

Published on: December 17, 2018

10.8K
Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures
09:12

Facet-to-facet Linking of Shape-anisotropic Colloidal Cadmium Chalcogenide Nanostructures

Published on: August 10, 2017

8.0K
The Measurement of Unsteady Surface Pressure Using a Remote Microphone Probe
08:53

The Measurement of Unsteady Surface Pressure Using a Remote Microphone Probe

Published on: December 3, 2016

7.4K

Area of Science:

  • Environmental Chemistry
  • Geochemistry
  • Microbial Ecology

Background:

  • Surface complexation modeling (SCM) uses thermodynamic stability constants to predict metal adsorption.
  • The component additivity (CA) approach extends SCM to complex multi-sorbent systems.
  • Quantitative testing of CA models is challenging due to difficulties in measuring metal distribution.

Purpose of the Study:

  • To quantitatively test the CA approach for predicting cadmium (Cd) adsorption in multi-sorbent systems.
  • To introduce and validate a novel Cd-specific fluorescent probe technique for measuring Cd distribution.
  • To assess the accuracy of CA predictions in the presence and absence of a chelating ligand (EDTA).

Main Methods:

  • Utilized a cadmium-specific fluorescent probe coupled with confocal scanning laser microscopy.
  • Quantified Cd adsorption to Bacillus subtilis bacterial cells and kaolinite in hydrated multi-sorbent samples.
  • Calculated Cd distribution using the CA approach and compared it with experimental measurements.

Main Results:

  • The CA approach accurately predicted Cd distribution between aqueous, bacterial, and mineral phases within experimental uncertainties.
  • Measurements were consistent in both EDTA-free and EDTA-containing systems.
  • The Cd-fluorescent probe technique proved effective for quantitative testing of CA models.

Conclusions:

  • The Cd-fluorescent probe technique is a viable method for validating surface complexation models.
  • The CA approach provides reasonable predictions for Cd distribution in mixed sorbent systems.
  • Prediction accuracy is contingent upon the precise measurement of stability constants for all metal-ligand complexes.