Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Co-activators and Co-repressors02:04

Co-activators and Co-repressors

8.6K
Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
8.6K
tRNA Activation02:26

tRNA Activation

23.0K
Aminoacyl-tRNA synthetases are present in both eukaryotes and bacteria. Though eukaryotes have 20 different aminoacyl-tRNA synthetases to couple to 20 amino acids, many bacteria do not have genes for all of these aminoacyl-tRNA synthetases. Despite this, they still use all 20 amino acids to synthesize their proteins. For instance, some bacteria do not have the gene encoding the enzyme that couples glutamine with its partner tRNA. In these organisms, one enzyme adds glutamic acid to all of the...
23.0K
Activation Energy01:26

Activation Energy

86.7K
Activation energy is the minimum amount of energy necessary for a chemical reaction to move forward. The higher the activation energy, the slower the rate of the reaction. However, adding heat to the reaction will increase the rate, since it causes molecules to move faster and increase the likelihood that molecules will collide. The collision and breaking of bonds represents the uphill phase of a reaction and generates the transition state. The transition state is an unstable high-energy state...
86.7K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.8K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.8K
Secondary Active Transport01:55

Secondary Active Transport

137.9K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme “pump” embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
137.9K
Primary Active Transport01:47

Primary Active Transport

198.9K
In contrast to passive transport, active transport involves a substance being moved through membranes in a direction against its concentration or electrochemical gradient. There are two types of active transport: primary active transport and secondary active transport. Primary active transport utilizes chemical energy from ATP to drive protein pumps that are embedded in the cell membrane. With energy from ATP, the pumps transport ions against their electrochemical gradients—a direction...
198.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

RIG-I-targeted immunotherapy synergizes with immune checkpoint inhibition in a hepatocellular carcinoma model.

Molecular cancer therapeutics·2026
Same author

Glucagon and beta-cell function changes before and after dietary weight loss-induced metabolic improvements in type 2 diabetes.

Diabetes research and clinical practice·2026
Same author

Targeting Inflammation by Pioglitazone and its R-Enantiomer Mitigates Pathological Myocardial Remodeling in Murine Hypertrophic Cardiomyopathy.

JACC. Basic to translational science·2026
Same author

Trace element dynamics following low-calorie formula diet and their association with glycaemic traits.

Clinical nutrition (Edinburgh, Scotland)·2026
Same author

Immune dyshomeostasis in lifestyle-associated diseases: a new paradigm for personalized medicine and prevention.

Current opinion in immunology·2026
Same author

Inflammasome adaptor ASC promotes sustained neuroinflammation and mild cognitive impairment in a closed-head injury model.

The Journal of clinical investigation·2026

Related Experiment Video

Updated: Feb 4, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.4K

NLRP3 inflammasome activation in inflammaging.

Eicke Latz1, Peter Duewell2

  • 1Institute of Innate Immunity, University of Bonn, 53127, Bonn, Germany; Department of Infectious Diseases and Immunology, University of Massachusetts Medical School, Worcester, Massachusetts, USA; German Center for Neurodegenerative Diseases (DZNE), Bonn, Germany.

Seminars in Immunology
|October 1, 2018
PubMed
Summary

Aging triggers inflammaging and metaflammation, driven by lifestyle factors. The NLRP3 inflammasome pathway recognizes these triggers, contributing to age-related diseases. Targeting this pathway may offer therapeutic strategies.

Keywords:
AgingInflammagingMetaflammationNLRP3 inflammasomeProinflammatory cytokines

More Related Videos

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

11.3K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.8K

Related Experiment Videos

Last Updated: Feb 4, 2026

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells
09:04

Activation and Measurement of NLRP3 Inflammasome Activity Using IL-1β in Human Monocyte-derived Dendritic Cells

Published on: May 22, 2014

21.4K
Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages
06:52

Detection of Inflammasome Activation and Pyroptotic Cell Death in Murine Bone Marrow-derived Macrophages

Published on: May 21, 2018

11.3K
Evaluation of Caspase Activation to Assess Innate Immune Cell Death
10:23

Evaluation of Caspase Activation to Assess Innate Immune Cell Death

Published on: January 20, 2023

3.8K

Area of Science:

  • Immunology
  • Gerontology
  • Metabolic Health

Background:

  • Aging is linked to chronic low-grade inflammation (inflammaging), accelerating age-related diseases.
  • Modern lifestyles and excess calorie intake exacerbate inflammation (metaflammation).

Purpose of the Study:

  • To review mechanisms driving inflammaging and metaflammation.
  • To highlight the role of the NLRP3 inflammasome in these processes.
  • To discuss therapeutic strategies targeting inflammation in aging.

Main Methods:

  • Literature review of current knowledge on inflammaging and metaflammation.
  • Focus on the NLRP3 inflammasome's role in recognizing aging and metabolic stress triggers.
  • Discussion of the link between these inflammatory processes and age-related pathologies.

Main Results:

  • The NLRP3 inflammasome is a key innate immune pathway involved in recognizing aging and metabolic stress.
  • Inflammaging and metaflammation, mediated by NLRP3, contribute to the pathogenesis of common age-related diseases.
  • Pharmacological targeting of NLRP3 inflammasome activation presents potential therapeutic avenues.

Conclusions:

  • The NLRP3 inflammasome is central to age-related inflammation and disease.
  • Understanding these pathways offers opportunities for interventions to mitigate age-related pathologies.
  • Targeting the NLRP3 inflammasome could be a strategy to address detrimental inflammatory responses in aging.