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Bacterial Detection & Identification Using Electrochemical Sensors
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Hybrid electrochemical sensor platform for capsaicin determination using coarsely stepped cyclic squarewave
Sindre Søpstad1, Kristin Imenes1, Erik A Johannessen1
1Department of Microsystems, Faculty of Technology, Maritime and Natural Sciences, University of South-Eastern Norway, Borre, Norway.
Abstract:
A small, standalone electrochemical hybrid sensor platform, combining flexible electronics and screen-printed electrodes, is demonstrated in the determination of capsaicin through adsorptive stripping voltammetry. The sensing scheme was simplified to be compatible with a low-cost device. The simplification involved eliminating the need for additional modification of the electrode and employing a coarsely stepped squarewave voltammetry, a technique which is applicable with less sophisticated instrumentation. This architecture was found to be suitable for concentrations up to at least 5000 µM with a detection limit of 1.98 µM. The screen-printed carbon graphite electrodes were made reusable through an ethanol rinsing protocol. The effect of ethanol/buffer volumetric ratio in the test sample was shown to greatly influence the analytical data, and a fixed 10% (v/v) was chosen as a compromise between signal-to-noise ratio and not exceeding the solubility limit of the desired upper range.
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