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Coincident gene conversion events in yeast that involve a large insertion.

J E Golin, S C Falco, J P Margolskee

    Genetics
    |December 1, 1986
    PubMed
    Summary

    Spontaneous gene conversion in yeast is frequent, possibly due to long DNA structures. This study tested a long heteroduplex model, finding frequent plasmid loss, suggesting alternative recombination models may be needed.

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    Area of Science:

    • Molecular Biology
    • Genetics
    • Yeast Genetics

    Background:

    • Spontaneous gene conversion at distant loci in yeast is unexpectedly frequent during mitosis.
    • A proposed explanation involves a long, continuous heteroduplex DNA intermediate.
    • The frequency is approximately 1000 times higher than expected for independent recombination events.

    Purpose of the Study:

    • To investigate the long heteroduplex model for frequent, coincident recombination events in yeast.
    • To examine the behavior of a large plasmid insertion within flanking recombination sites.
    • To determine if the observed recombination patterns support or refute the heteroduplex model.

    Main Methods:

    • Introduction of a large plasmid insertion between the LEU1 and TRP5 loci in yeast via transformation.
    • Analysis of coincident gene conversion events involving the flanking sites.
    • Assessment of plasmid stability and loss within convertant populations.

    Main Results:

    • Frequent loss of the plasmid was observed in convertants when it was in hemizygous or homozygous configuration.
    • These findings challenge the straightforward application of the long heteroduplex model.
    • The results necessitate new assumptions about DNA mismatch formation and resolution.

    Conclusions:

    • The study provides data that could support the long heteroduplex model for coincident recombination, but requires novel assumptions.
    • An alternative model proposing multiple, concerted recombination events is discussed as a potential explanation.
    • Further research is needed to elucidate the precise mechanisms of distant gene conversion in yeast.

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