Related Experiment Video
Updated: Dec 31, 2025
Quantification of Reactive Oxygen Species Using 2′,7′-Dichlorofluorescein Diacetate Probe and Flow-Cytometry in Müller Glial Cells
Published on: May 13, 2022
Thymoquinone protects human retinal pigment epithelial cells against hydrogen peroxide induced oxidative stress and
Xin Hu1, Yuanyuan Liang1, Bo Zhao1
1Department of Ophthalmology, Huaihe Hospital, Henan University, Kaifeng, China.
Abstract:
Oxidative stress in retinal pigment epithelium (RPE) cells may contribute to the progression of age-related macular degeneration. Thymoquinone (TQ), an active component derived from Nigella sativa, possesses antioxidative effect. However, the role of TQ in RPE cells under oxidative stress condition remains unclear. The present study aimed to examine the protective effect of TQ against hydrogen peroxide (H2 O2 )-induced oxidative stress in human RPE cells. Our results showed that TQ improved the cell viability and apoptosis in H2 O2 -induced ARPE cells. We also found that the levels of reactive oxygen species and malondialdehyde induced by H2 O2 were reduced after the pretreatment of TQ. In addition, the inhibitory effect of H2 O2 on the glutathione (GSH) level and superoxide dismutase activity was markedly attenuated by TQ pretreatment. Moreover, TQ enhanced the activation of Nrf2/heme oxygenase 1 (HO-1) signaling pathway in H2 O2 -induced ARPE cells. Knockdown of Nrf2 abolished the protective effect of TQ on H2 O2 -induced oxidative damage. These results suggested that TQ protected ARPE cells from H2 O2 -induced oxidative stress and apoptosis via the Nrf2/HO-1 signaling pathway.
More Related Videos
08:04Cone-Enriched Cultures from the Retina of Chicken Embryos to Study Rod to Cone Cellular Interactions
Published on: March 20, 2021
10:00Induction and Analysis of Oxidative Stress in Sleeping Beauty Transposon-Transfected Human Retinal Pigment Epithelial Cells
Published on: December 11, 2020