Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

14.6K
Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.6K
Conservation of Protein Domains02:26

Conservation of Protein Domains

4.1K
4.1K
Conserved Binding Sites01:49

Conserved Binding Sites

5.2K
Many proteins’ biological role depends on their interactions with their ligands, small molecules that bind to specific locations on the protein known as ligand-binding sites. Ligand-binding sites are often conserved among homologous proteins as these sites are critical for protein function.
Binding sites are often located in large pockets, and if their location on a protein’s surface is unknown, it can be predicted using various approaches. The energetic method computationally...
5.2K
What is Conservation Biology?01:57

What is Conservation Biology?

24.4K
Conservation biology is a scientific field that focuses on the preservation of biodiversity in order to protect ecosystems while meeting the needs of the human population. Humans require properly functioning ecosystems to maintain our supply of natural resources, including food, medicines, and building materials.
24.4K
Conservation of Small Populations02:04

Conservation of Small Populations

17.4K
Small population sizes put a species at extreme risk of extinction due to a lack of variation, and a consequent decrease in adaptability. This weakens the chances of survival under pressures such as climate change, competition from other species, or new diseases. Large populations are more likely to survive pressures such as these, as such populations are more likely to harbor individuals that have genetic variants that are adaptive under new stresses. Small populations are much less...
17.4K
Non-conservative Forces01:17

Non-conservative Forces

9.9K
Non-conservative forces are dissipative forces such as friction or air resistance. These forces take energy away from a system as it progresses. Unlike conservative forces, non-conservative forces do not have potential energy associated with them. This is because the energy is lost to the system and cannot be turned into useful work later.
Also unlike their conservative counterparts, they are path-dependent; where the object starts and stops does matter. For example, a grinding wheel applies a...
9.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structural equation modeling of exercise intention in patients recovering from stroke: a cross-sectional study.

Scientific reports·2026
Same author

Efficacy and Safety of Secukinumab in Enthesitis-Related Arthritis: A Retrospective Study in China.

International journal of rheumatic diseases·2026
Same author

Integration of single-cell transcriptome of female early gonadal development in bovine, goats, and pigs.

iScience·2026
Same author

Expanding the Gene Expression Profiling of Drug Transporters and Drug-Metabolizing Enzymes to Include the Upper Female Reproductive Tract.

Pharmaceutics·2026
Same author

In Vitro Pharmacokinetic Properties of MK-2048, a Potent Drug Candidate for HIV Prevention.

Viruses·2026
Same author

The efficacy and safety of metronomic vinorelbine combined with anlotinib in HER2-negative advanced breast cancer: a prospective, single-arm clinical study.

Translational breast cancer research : a journal focusing on translational research in breast cancer·2026

Related Experiment Video

Updated: Feb 4, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.4K

Protein AMPylation by an Evolutionarily Conserved Pseudokinase.

Anju Sreelatha1, Samantha S Yee1, Victor A Lopez1

  • 1Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.

Cell
|October 2, 2018
PubMed
Summary
This summary is machine-generated.

Pseudokinases, once thought inactive, can transfer AMP to proteins. Selenoprotein-O (SelO) exhibits this AMPylation activity, crucial for cellular response to oxidative stress.

Keywords:
SELENOOadenylylationglutaredoxinglutathionylationkinase structureoxidative stressselenocysteine

More Related Videos

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
16:02

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

Published on: February 10, 2023

3.3K
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
12:23

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

8.0K

Related Experiment Videos

Last Updated: Feb 4, 2026

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays
03:09

Author Spotlight: Advancing Structural and Biochemical Studies of Proteins Through Thermal Shift Assays

Published on: August 9, 2024

1.4K
Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation
16:02

Demonstration of the Sequence Alignment to Predict Across Species Susceptibility Tool for Rapid Assessment of Protein Conservation

Published on: February 10, 2023

3.3K
Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
12:23

Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG

Published on: May 16, 2017

8.0K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Biology

Background:

  • Protein kinases are crucial enzymes, but ~10% are classified as pseudokinases due to lacking catalytic residues.
  • Pseudokinases were largely considered catalytically inactive, limiting their known functional roles.

Purpose of the Study:

  • To investigate the functional activity of the conserved pseudokinase, selenoprotein-O (SelO).
  • To explore potential enzymatic activities beyond canonical kinase function for pseudokinases.

Main Methods:

  • X-ray crystallography to determine the structural basis of SelO activity.
  • In vitro assays to demonstrate AMP transfer to protein substrates.
  • Cellular localization studies and analysis of SelO's role in oxidative stress response.

Main Results:

  • SelO catalyzes the transfer of AMP from ATP to Ser, Thr, and Tyr residues on protein substrates, a process termed AMPylation.
  • Crystal structure reveals a kinase-like fold with an altered ATP binding site, explaining the catalytic mechanism.
  • SelO localizes to mitochondria and AMPylates proteins regulating redox homeostasis, demonstrating its role in cellular defense.

Conclusions:

  • Pseudokinases, exemplified by SelO, possess novel transferase activities like AMPylation.
  • AMPylation is a potentially widespread post-translational modification.
  • SelO's activity is vital for cellular adaptation to oxidative stress, highlighting pseudokinases as functional enzymes.