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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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Lateralization01:28

Lateralization

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Brain lateralization refers to the division of mental processes and functions between the two hemispheres of the brain, a phenomenon that optimizes neural efficiency and underpins complex abilities in humans. This specialization allows each hemisphere to perform tasks where it has a comparative advantage, facilitating more refined cognitive capabilities across different domains.
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Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

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Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
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Multiple Allele Traits01:49

Multiple Allele Traits

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The Concept of Multiple Allelism
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Cranial Bones: Lateral View01:27

Cranial Bones: Lateral View

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The lateral view of the cranium is dominated by temporal, sphenoid, and ethmoid bones.
The temporal bone forms the lower lateral side of the skull. The temporal bone is subdivided into several regions. The flattened upper portion is the squamous portion of the temporal bone. Below this area and projecting anteriorly is the zygomatic process of the temporal bone, which forms the posterior portion of the zygomatic arch. Posteriorly is the mastoid portion of the temporal bone. Projecting...
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Related Experiment Video

Updated: Feb 4, 2026

Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Visual Detection of Multiple Nucleic Acids in a Capillary Array

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Lateral Flow Test for Visual Detection of Multiple MicroRNAs.

Wanli Zheng1, Li Yao1, Jun Teng1

  • 1School of Food Science & Engineering, Hefei University of Technology, 230009, Anhui, PR China.

Sensors and Actuators. B, Chemical
|October 2, 2018
PubMed
Summary

This study presents a rapid, low-cost multiplex assay for detecting multiple microRNAs (miRNAs) using a lateral flow nucleic acid biosensor. The developed biosensor offers a valuable tool for clinical applications and biomedical diagnosis, especially in resource-limited settings.

Keywords:
Lateral flow biosensormicroRNAmultiplex detectionserum samplesimultaneous detection

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Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
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Visual Detection of Multiple Nucleic Acids in a Capillary Array
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Detection of MicroRNA Expression in the Kidneys of Immunoglobulin A Nephropathic Mice
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Area of Science:

  • Biomedical Diagnostics
  • Biosensor Technology
  • Nucleic Acid Assays

Background:

  • MicroRNA (miRNA) detection is crucial for biomedical diagnosis.
  • Existing miRNA assays can be costly and time-consuming.
  • There is a need for rapid, low-cost, and multiplexed diagnostic tools.

Purpose of the Study:

  • To develop a rapid and low-cost multiplex microRNA assay.
  • To utilize a lateral flow nucleic acid biosensor (LFNAB) for simultaneous miRNA detection.
  • To validate the assay's performance in complex biological samples like serum.

Main Methods:

  • Employing sandwich-type nucleic acid hybridization for complex formation.
  • Utilizing gold nanoparticles (GNPs) as visual tags for detection.
  • Designing a multiplex LFNAB with three distinct test zones for simultaneous detection of specific miRNAs (miR-21, miR-155, miR-210).

Main Results:

  • Achieved simultaneous detection of three target microRNAs (miRNA-21, miRNA-155, miRNA-210).
  • Demonstrated successful application of the assay in spiked serum samples with satisfactory results.
  • Validated the efficacy of the GNP-based visual tag and adding-measuring model.

Conclusions:

  • The developed LFNAB offers a rapid, low-cost, and multiplexed approach for miRNA detection.
  • This assay holds significant potential for clinical applications and biomedical diagnosis.
  • The method is particularly valuable for resource-limited settings requiring efficient diagnostic tools.