Detection of infectious pancreatic necrosis virus using an inhibition enzyme-linked immunosorbent assay

An inhibition enzyme-linked immunosorbent assay was used to detect infectious pancreatic necrosis (IPN) virus. In this assay the presence of virus was determined by measuring the decrease in titer of a known antiserum after incubation with a sample suspected to contain virus. The titer of the antiserum was measured with an indirect enzyme-linked assay. Compared to the double antibody sandwich method this assay required fewer reagents (only one anti-IPN serum was required). This assay was also sensitive enough to detect virus at levels of 1 X 10(2) TCID 50/ml. of purified virus and was able to detect virus in samples obtained in the field.