Structural basis for promotion of duodenal iron absorption by enteric ferric reductase with ascorbate

Dietary iron absorption is regulated by duodenal cytochrome b (Dcytb), an integral membrane protein that catalyzes reduction of nonheme Fe³⁺ by electron transfer from ascorbate across the membrane. This step is essential to enable iron uptake by the divalent metal transporter. Here we report the crystallographic structures of human Dcytb and its complex with ascorbate and Zn²⁺. Each monomer of the homodimeric protein possesses cytoplasmic and apical heme groups, as well as cytoplasmic and apical ascorbate-binding sites located adjacent to each heme. Zn²⁺ coordinates to two hydroxyl groups of the apical ascorbate and to a histidine residue. Biochemical analysis indicates that Fe³⁺ competes with Zn²⁺ for this binding site. These results provide a structural basis for the mechanism by which Fe³⁺ uptake is promoted by reducing agents and should facilitate structure-based development of improved agents for absorption of orally administered iron.