Related Experiment Video

Updated: Feb 4, 2026

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
06:32

Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures

Published on: January 9, 2019

8.3K

Anti-metastatic effect of 131I-labeled Buthus martensii Karsch chlorotoxin in gliomas

Shan Wu1, Ke Ma2, Wen-Li Qiao3

  • 1Department of Nuclear Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, P.R. China.

International Journal of Molecular Medicine
|October 2, 2018
PubMed

Insights

Buthus martensii Karsch chlorotoxin (BmK CT) inhibits glioma cell invasion and metastasis by regulating tissue inhibitor of metalloproteinase-2. Radiolabeled 131I-BmK CT shows potential as a targeted glioma therapy.

Area of Science:

  • Molecular mechanisms of cancer progression
  • Neurotoxins and their therapeutic applications
  • Radioisotope-based cancer therapy

Background:

  • Glioma invasion and metastasis are critical challenges in treatment.
  • Targeted therapies are needed to improve patient outcomes.
  • Buthus martensii Karsch chlorotoxin (BmK CT) is a potential anti-cancer agent.

Purpose of the Study:

  • To elucidate the molecular mechanism of BmK CT in inhibiting glioma cell invasion and metastasis.
  • To evaluate 131I-labeled BmK CT (131I-BmK CT) as a potential targeted therapeutic agent for glioma.

Main Methods:

  • In vitro assays including Cell Counting Kit-8, colony formation, Transwell invasion, and wound-healing assays.
  • Protein expression analysis using Western blotting, ELISA, immunofluorescence, and gelatin zymography.
  • Assessment of BmK CT and 131I-BmK CT effects on glioma cells.

Main Results:

  • BmK CT significantly inhibited glioma cell invasion and metastasis.
  • The inhibitory effect was mediated by the regulation of tissue inhibitor of metalloproteinase-2 expression.
  • 131I-BmK CT demonstrated cytotoxic effects and maintained inhibitory properties in vitro.

Conclusions:

  • BmK CT effectively suppresses glioma cell invasion and metastasis through modulation of tissue inhibitor of metalloproteinase-2.
  • 131I-BmK CT represents a promising novel targeted therapeutic agent for glioma treatment.

More Related Videos

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates08:32

Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array HMCA-based Plates

Published on: October 25, 2018

9.5K
Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window09:44

Imaging Glioma Initiation In Vivo Through a Polished and Reinforced Thin-skull Cranial Window

Published on: November 20, 2012

13.7K

Related Concept Videos

Labeling Emotion01:20

Labeling Emotion

Emotional labeling is a cognitive process that involves identifying and naming one's emotions, such as anger, fear, happiness, or sadness. It allows individuals to recognize and express their internal emotional states, a critical aspect of emotional regulation and communication. Labeling emotions requires more than mere recognition; it also involves drawing upon memory and contextual cues to understand the current situation and apply a corresponding emotional label. For instance, feeling...
731
Waterproofing and Anti-Bacterial Admixtures in Concrete01:22

Waterproofing and Anti-Bacterial Admixtures in Concrete

Concrete's susceptibility to water absorption is due to the capillary action within the pores of its hydrated cement paste. This action draws water in, creating the need for waterproofing admixtures to prevent such penetration. The efficacy of these admixtures is contingent upon the water pressure, with variations arising from different conditions such as rain, capillary rise, or hydrostatic pressure in structures intended to hold water.
Waterproofing admixtures render concrete hydrophobic,...
242
Labeling DNA Probes03:31

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
9.4K
Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids02:04

Oxidation of Alkenes: Anti Dihydroxylation with Peroxy Acids

Diols are compounds with two hydroxyl groups. In addition to syn dihydroxylation, diols can also be synthesized through the process of anti dihydroxylation. The process involves treating an alkene with a peroxycarboxylic acid to form an epoxide. Epoxides are highly strained three-membered rings with oxygen and two carbons occupying the corners of an equilateral triangle. This step is followed by ring-opening of the epoxide in the presence of an aqueous acid to give a trans diol.
7.4K
Radical Anti-Markovnikov Addition to Alkenes: Overview01:25

Radical Anti-Markovnikov Addition to Alkenes: Overview

The addition of hydrogen bromide to alkenes in the presence of hydroperoxides or peroxides proceeds via an anti-Markovnikov pathway and yields alkyl bromides.
4.3K
Radical Anti-Markovnikov Addition to Alkenes: Thermodynamics01:32

Radical Anti-Markovnikov Addition to Alkenes: Thermodynamics

The anti-Markovnikov addition of hydrogen halides to an alkene is thermodynamically feasible only with HBr. The radical addition reaction with other hydrogen halides like HCl and HI is thermodynamically unfavorable.
2.7K