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Published on: July 9, 2011
First Report on Detection of Dengue Virus in the Donor Cornea
Madhuravasal Krishnan Janani1, Parthasarathy Durgadevi, Jayavel Padmapriya
1Larsen & Toubro Microbiology Research Centre, Vision Research Foundation, Chennai, India.
Background:
Dengue fever is the most prevalent form of flavivirus infection in humans. We have investigated whether corneoscleral tissue of the donor affected by dengue virus (DENV) harbors the virus.
Purpose:
To identify the risk for viral transmission through corneal transplants in areas where DENV circulates.
Methods:
Excised corneoscleral tissue from a cadaver with a history of viral hemorrhagic fever was analyzed using reverse transcriptase-polymerase chain reaction for the presence of DENV and chikungunya virus (CHIV).
Results:
DENV was detected in RNA extracted from the donor corneoscleral rim. Further genotyping of the viral isolate from the virus-infected cell harvest revealed DENV type 3 as the causative agent. CHIV was not detected.
Conclusions:
The data presented in this study recommend the implementation of polymerase chain reaction for detection of DENV and CHIV to analyze excised corneoscleral tissue of a donor with viral hemorrhagic fever.
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