Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

In-situ Hybridization02:31

In-situ Hybridization

10.6K
In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...
10.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.6K
5.6K
Profile Leveling and Cross Sections01:26

Profile Leveling and Cross Sections

1.7K
Profile leveling and cross-sections are surveying methods used to determine and document terrain elevations for infrastructure projects such as highways, railroads, canals, and pipelines. These methods provide data for earthwork planning and alignment of proposed routes.  Profile leveling involves measuring elevations along a fixed line to create a vertical terrain profile. A surveyor sets up a leveling instrument at the benchmark (BM) and records a backsight (BS) to determine the...
1.7K
FISH - Fluorescent In-situ Hybridization02:07

FISH - Fluorescent In-situ Hybridization

24.5K
Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
24.5K
Hybrid Zones02:29

Hybrid Zones

21.9K
Hybrid zones are narrow regions where two closely related species interact, mate, and produce hybrids. Relative to either parent species, hybrids may possess distinct phenotypic or genetic differences that impact their survival and reproductive success. The genetic variances introduced by hybridization influence species diversity and speciation processes within the hybrid zone.
21.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Defining Exercise Hemodynamics and Function After Transcatheter Aortic Valve Replacement: The DEFINE-TAVR Prospective Study.

Journal of the Society for Cardiovascular Angiography & Interventions·2026
Same author

Loss of Mtarc1 Protects Against Steatotic Liver Disease in Mice.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

A spatial transcriptomic atlas of autism-associated genes identifies convergence in the developing human thalamus.

bioRxiv : the preprint server for biology·2025
Same author

Safety and Efficacy of Endovascular Treatment Modalities for Below-the-Knee Arterial Disease: A Systematic Review and Network Meta-analysis.

Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists·2025
Same author

Cell-intrinsic metabolic phenotypes identified in patients with glioblastoma, using mass spectrometry imaging of <sup>13</sup>C-labelled glucose metabolism.

Nature metabolism·2025
Same author

Quantitative characterization of cell niches in spatially resolved omics data.

Nature genetics·2025

Related Experiment Video

Updated: Feb 4, 2026

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.6K

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization.

Fani Memi1, Daniela Tirziu2, Irinna Papangeli3

  • 1Department of Cell and Developmental Biology, University College London.

Journal of Visualized Experiments : Jove
|October 2, 2018
PubMed
Summary

This study presents a method for detecting micro-RNAs (miRNAs) and proteins in mouse heart tissue. The technique uses digoxigenin-labeled Locked Nucleic Acid probes for miRNA detection and protein immunostaining for co-localization.

More Related Videos

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

7.1K
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
09:23

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

14.5K

Related Experiment Videos

Last Updated: Feb 4, 2026

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization
08:22

Tissue-specific miRNA Expression Profiling in Mouse Heart Sections Using In Situ Hybridization

Published on: September 15, 2018

8.6K
Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization
06:01

Clinicopathological Analysis of miRNA Expression in Breast Cancer Tissues by Using miRNA In Situ Hybridization

Published on: June 7, 2016

7.1K
Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections
09:23

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

Published on: March 26, 2016

14.5K

Area of Science:

  • Molecular Biology
  • Cardiovascular Research
  • Biochemistry

Background:

  • Micro-RNAs (miRNAs) are crucial regulators of gene expression involved in numerous biological and disease processes.
  • Reliable methods for detecting specific miRNA transcripts in tissue are essential for understanding their roles.
  • Cardiac hypertrophy involves complex molecular changes, necessitating tools to study miRNA involvement.

Purpose of the Study:

  • To establish a detailed protocol for simultaneous detection of miRNA and protein expression in mouse heart sections.
  • To validate the protocol using miRNA-182 and cardiac Troponin T (cTnT) in a cardiac hypertrophy model.
  • To provide a versatile method for investigating miRNA and protein co-localization in cardiovascular research.

Main Methods:

  • In situ hybridization using digoxigenin-labeled (DIG) Locked Nucleic Acid (LNA) probes for miRNA detection.
  • Immunohistochemistry for cardiac Troponin T (cTnT) protein detection on the same tissue sections.
  • Combined colorimetric (alkaline phosphatase) and fluorescent assays for simultaneous signal visualization.

Main Results:

  • Successfully detected miRNA-182 expression in mouse heart sections using DIG-LNA probes.
  • Co-localized miRNA-182 with cardiomyocytes by immunostaining for cTnT protein.
  • Demonstrated the feasibility of the combined protocol for simultaneous miRNA and protein analysis.

Conclusions:

  • The developed protocol enables sensitive and specific detection of miRNAs and proteins in mouse heart tissue.
  • This method facilitates the study of miRNA roles in cardiac diseases by allowing co-localization with specific cell types or proteins.
  • The protocol is adaptable for detecting various miRNAs and proteins, offering a valuable tool for cardiovascular research.