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Updated: Feb 4, 2026

Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Published on: September 13, 2018
Genome-wide Surveillance of Transcription Errors in Eukaryotic Organisms
Clark Fritsch1, Jean-Francois Pierre Gout2, Marc Vermulst3
1Center for Mitochondrial and Epigenomic Medicine, Children's Hospital of Philadelphia; Department of Cellular and Molecular Biology, University of Pennsylvania.
Abstract:
Accurate transcription is required for the faithful expression of genetic information. Surprisingly though, little is known about the mechanisms that control the fidelity of transcription. To fill this gap in scientific knowledge, we recently optimized the circle-sequencing assay to detect transcription errors throughout the transcriptome of Saccharomyces cerevisiae, Drosophila melanogaster, and Caenorhabditis elegans. This protocol will provide researchers with a powerful new tool to map the landscape of transcription errors in eukaryotic cells so that the mechanisms that control the fidelity of transcription can be elucidated in unprecedented detail.
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