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Updated: Feb 4, 2026

Co-expression of Multiple Chimeric Fluorescent Fusion Proteins in an Efficient Way in Plants
Published on: July 1, 2018
Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A
Yuan Zong1,2, Qianna Song1,2, Chao Li1,2
1State Key Laboratory of Plant Cell and Chromosome Engineering, Center for Genome Editing, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
Abstract:
Base editors (BEs) have been used to create C-to-T substitutions in various organisms. However, editing with rat APOBEC1-based BE3 is limited to a 5-nt sequence editing window and is inefficient in GC contexts. Here, we show that a base editor fusion protein composed of Cas9 nickase and human APOBEC3A (A3A-PBE) converts cytidine to thymidine efficiently in wheat, rice and potato with a 17-nucleotide editing window at all examined sites, independent of sequence context.
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