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Tracing the protective activity of Lactobacillus plantarum using technetium-99m-labeled zearalenone for organ
M H Sanad1, A S M Fouzy2, Hassan M Sobhy3
1a Labeled Compound Department, Radioisotopes Production and Radioactive Sources Division , Hot Lab. Centre, Atomic Energy Authority , Cairo , Egypt.
Purpose:
Zearalenone is a potent estrogenic metabolite produced by some Fusarium and Gibberella species. Therefore, the aim of this study was to determine the accumulation of Technetium-[99m]-labeled zearalenone in mice organs as well as to study the protective effect of Lactobacillus plantarum.
Materials And Methods:
Two different groups of mice were used; the first group of mice received Technetium-[99m]-zearalenone only, and the second group received both Technetium-[99m] zearalenone and L. plantarum (as a protective agent). Technetium-[99m]-labeled zearalenone was administered to mice by tail vein injection and L. plantarum was administered orally. Accumulation of labeled zearalenone in mice was monitored for 120 min.
Results:
Labeled zearalenone was distributed primarily in the intestine (44.5%) and liver (20.12%) after 120 min in the first group of mice. In the second group of mice which received both Technetium-[99m] zearalenone and L. plantarum, labeled zearalenone was accumulated primarily in the intestine (46.8%) and liver (18.9%). These results indicated the decrease in labeled zearalenone in the liver due to the effect of lactic acid bacteria.
Conclusion:
Technetium-[99m]-labeled zearalenone can be used as a new tracer for organ imaging and that L. plantarum can decrease the bioavailability of zearalenone in mice organs.
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