Antibacterial Surface Coating for Bone Scaffolds Based on the Dark Catalytic Effect of Titanium Dioxide

Biomaterials which promote tissue integration and resist microbial colonisation are required in bone tissue engineering to prevent biomaterial-associated infections. Surface modification of established materials for bone tissue engineering, such as TiO₂, have emerged as promising anti-infective strategies. Interestingly, the antibacterial activity of TiO₂ in the form of particles can be enhanced by combining it with H₂O₂, even in the absence of irradiation. However, it remains unknown whether TiO₂ surfaces elicit a similar effect. In this study, the antibacterial effect of porous TiO₂ scaffolds generated by the catalytic decomposition of H₂O₂ in the absence of light (dark catalysis) was investigated. Porous ceramic foams were fabricated and sol-gel coated for high catalytic activity. Degradation of methylene blue in the presence of 3% H₂O₂ increased by 80% for the sol-gel-coated surfaces. The degradation kinetics indicate that intermediate free radicals that form at the liquid-TiO₂ interface are responsible for the oxidative behavior of the surface. TiO₂ surfaces were further pretreated with 30% H₂O₂ for prolonged oxidative behavior. The biological response toward such surfaces was assessed in vitro. S. epidermidis biofilms formed on modified surfaces showed reduced viability compared to nonmodified surfaces. Further, the same surface modification showed no cytotoxic effects on MC3T3 preosteoblasts. However, the results from the conducted genotoxicity assay were inconclusive, and further studies are needed to exclude ROS-mediated DNA damage. To conclude, this study provides evidence that a simple surface modification based on the dark catalytic effect of TiO₂ can be used to create antibacterial surface properties for ceramic bone scaffolds.