Effect of heat-transfer capability on micropore structure of freeze-drying alginate scaffold

Biodegradable porous scaffold is widely used in the field of tissue engineering. Scaffolds were required to have proper microstructure with suitable size, gradient, shape and porosity to mimic the specific three-dimensional structure of extracellular matrix. Taken the simple technique of freeze-drying, the nucleation and growth of ice crystals were affected by thermal behavior of solution during freezing process, which could be controlled to tailor and predict the pore structure in scaffold. This paper aimed to reveal the effect of containers' heat-transfer capability on the thermal behaviors of solution, and then its influence to pore structure in scaffold. Four containers with different heat transfer capacity were adopted for cooling model to achieve different thermal characterizations within solution during cooling process. The vertical cross-section of scaffold was observed by scanning electron microscopy. The porosity and tensile properties of scaffold were measured to depict scaffold characteristics. Scaffolds fabricated by containers with λ/d = 20,000, 3000 and 450 W/m²·°C showed cellular pores with gradient. With the decrease of λ/d, pores in scaffold were enlarged, and the wall between neighboring pores also became thicker. Pillared pores were produced only in container with λ/d = 50 W/m²·°C, oriented along the vertical direction without gradient. This process demonstrated that by altering the container of cooling model, scaffold microstructure could be tailored.