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LP1 from Lentinula edodes C91-3 Induces Autophagy, Apoptosis and Reduces Metastasis in Human Gastric Cancer Cell Line

Samana Batool1, Thomson Patrick Joseph2, Mushraf Hussain3

  • 1Department of Microbiology, College of Basic Medical Sciences, Dalian Medical University, Dalian 116044, China. samana.batool12@yahoo.com.

International Journal of Molecular Sciences
|October 3, 2018
PubMed

Insights

Latcripin 1 (LP1) from mushrooms induces autophagy and apoptosis in gastric cancer cells. This natural compound shows potential as an anti-cancer agent by regulating cell death pathways and inhibiting migration.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Pharmacology

Background:

  • Gastric cancer remains a significant global health challenge, necessitating novel therapeutic strategies.
  • Natural products are a promising source for discovering new anti-cancer agents.
  • Latcripin 1 (LP1) from *Lentinula edodes* mushroom presents a potential candidate for gastric cancer treatment.

Purpose of the Study:

  • To investigate the anti-cancer effects of Latcripin 1 (LP1) on gastric cancer cell lines.
  • To elucidate the molecular mechanisms underlying LP1's action, including autophagy, apoptosis, and cell cycle regulation.
  • To assess LP1's impact on gastric cancer cell migration and invasion.

Main Methods:

  • Cell viability assays (CCK-8) and morphological observation.
  • Autophagy assessment using transmission electron microscopy and fluorescence microscopy.
  • Flow cytometry for apoptosis and cell cycle analysis.
  • Wound-healing, Transwell migration, and invasion assays.
  • Western blotting to analyze protein expression (autophagy-related genes, apoptosis markers, MMP-2/9, Akt/mTOR pathway).

Main Results:

  • LP1 induced autophagy, evidenced by autophagosome formation and LC3I/LC3II conversion.
  • LP1 upregulated autophagy-related genes (Atg7, Atg5, Atg12, Atg14, Beclin1) and promoted apoptosis via Bax/Bcl-2 modulation and Caspase-3 activation.
  • LP1 caused S-phase cell cycle arrest, decreased MMP-2/MMP-9 expression, and regulated the Akt/mTOR pathway.
  • LP1 inhibited gastric cancer cell migration and invasion.

Conclusions:

  • Latcripin 1 (LP1) exhibits significant anti-cancer properties against gastric cancer cells through the induction of autophagy and apoptosis.
  • LP1 modulates key molecular pathways, including cell cycle regulation, matrix metalloproteinase activity, and the Akt/mTOR signaling pathway.
  • LP1 demonstrates potential as a natural anti-cancer therapeutic agent for gastric cancer, warranting further in vitro and in vivo investigation.
Keywords:
Latcripin 1Lentinula edodesapoptosisautophagygastric cancermetastasis

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