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Updated: Feb 4, 2026

A Mouse Model for Laser-induced Choroidal Neovascularization
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A Mouse Model for Laser-induced Choroidal Neovascularization

Published on: December 27, 2015

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Inhibition of Experimental Choroidal Neovascularization by a Novel Peptide Derived from Calreticulin Anti-Angiogenic

Youn-Shen Bee1,2,3, Yi-Ling Ma4, Jinying Chen5,6

  • 1Department of Ophthalmology, Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan. ysbee@vghks.gov.tw.

International Journal of Molecular Sciences
|October 3, 2018
PubMed

Insights

A novel peptide, CAD27, effectively reduced choroidal neovascularization (CNV) in preclinical models. This peptide shows promise as a therapeutic for vision-threatening conditions like age-related macular degeneration.

Area of Science:

  • Ophthalmology
  • Angiogenesis Research
  • Drug Discovery

Background:

  • Choroidal neovascularization (CNV) is a critical factor in vision loss, notably in neovascular age-related macular degeneration.
  • Current treatments for CNV have limitations, necessitating novel therapeutic strategies.

Purpose of the Study:

  • To evaluate the anti-angiogenic potential of a calreticulin anti-angiogenic domain (CAD)-like peptide 27 (CAD27).
  • To assess CAD27's efficacy in inhibiting choroidal neovascularization in a preclinical rat model.

Main Methods:

  • In vitro assessment of CAD27's effects on endothelial cell tube formation, migration, and aortic ring sprouting.
  • In vivo evaluation of CAD27's impact on laser-induced CNV in rats via intravitreal injection and topical application.
  • Assessment of retinal function using electroretinography.

Main Results:

  • CAD27 significantly inhibited in vitro angiogenic activities.
  • Intravitreal injection of CAD27 reduced CNV lesions by up to 22.4%.
  • Topical application of CAD27 also reduced CNV lesions by up to 32.5%, with no adverse effects on retinal function.

Conclusions:

  • CAD27 demonstrates potent anti-angiogenic properties and effectively reduces CNV in a preclinical model.
  • CAD27 represents a promising therapeutic candidate for treating CNV in conditions like neovascular age-related macular degeneration.
Keywords:
calreticulin anti-angiogenic domainchoroidal neovascularizationneovascular age-related macular degeneration

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