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Transposable multiple antibiotic resistance in Streptococcus pneumoniae.

P Courvalin, C Carlier

    Molecular & General Genetics : MGG
    |November 1, 1986
    PubMed
    Summary

    A novel mobile genetic element, Tn1545, was discovered in antibiotic-resistant Streptococcus pneumoniae. This conjugative transposon confers resistance to multiple antibiotics and can transfer between bacterial species, aiding rapid spread.

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    Area of Science:

    • Molecular Biology
    • Microbiology
    • Genetics

    Background:

    • Antibiotic resistance is a growing global health threat.
    • Mobile genetic elements play a crucial role in the dissemination of antibiotic resistance genes.
    • Streptococcus pneumoniae is a significant human pathogen often exhibiting multidrug resistance.

    Purpose of the Study:

    • To characterize a novel mobile genetic element, Tn1545, found in a multidrug-resistant Streptococcus pneumoniae isolate.
    • To investigate the genetic basis of antibiotic resistance conferred by Tn1545.
    • To determine the transferability and transposition capabilities of Tn1545 in various bacterial hosts.

    Main Methods:

    • Isolation and characterization of the mobile genetic element Tn1545 from Streptococcus pneumoniae BM4200.
    • Molecular analysis to identify resistance genes (aphA-3, ermAM, tetM) within Tn1545.
    • Conjugation experiments using a recombination-deficient Enterococcus faecalis strain to assess Tn1545 transferability and transposition.

    Main Results:

    • Tn1545 is a 25.3 kb mobile genetic element conferring resistance to kanamycin (aphA-3), macrolides (ermAM), and tetracycline (tetM).
    • Tn1545 demonstrated self-transferability and transposition to various genomic sites in a recombination-deficient Enterococcus faecalis strain.
    • The element successfully conjugated and transposed into the chromosomes of multiple Gram-positive bacteria, including Streptococcus species, Staphylococcus aureus, and Listeria monocytogenes.

    Conclusions:

    • Tn1545 is a conjugative transposon capable of mediating the spread of multidrug resistance among diverse bacterial species.
    • The properties of Tn1545 provide a potential mechanism for the rapid emergence and dissemination of antibiotic resistance in Streptococcus pneumoniae, independent of plasmids.
    • Understanding the dynamics of mobile genetic elements like Tn1545 is critical for combating the spread of antibiotic resistance.

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