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In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice
07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

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miR-3148 Is a Novel Onco-microRNA that Potentiates Tumor Growth In Vivo

Takaki Akamine1,2, Yosuke Morodomi1,2, Yui Harada2

  • 1Department of Surgery and Science, Graduate School of Medical Sciences, Kyushu University, Fukuoka, Japan.

Anticancer Research
|October 3, 2018
PubMed
Abstract

Insights

MicroRNA-3148 (miR-3148) promotes colon cancer cell survival under stress conditions, particularly in vivo. This novel onco-microRNA may offer a new therapeutic target for cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Investigating microRNA (miRNA) expression in three-dimensional (3D) spheroids is crucial for identifying novel tumorigenesis markers.
  • Colon cancer cell behavior in 3D models can differ significantly from 2D cultures.

Purpose of the Study:

  • To identify novel microRNAs associated with tumorigenesis by examining miRNA expression in 3D spheroids.
  • To characterize the role of candidate onco-microRNAs in colon cancer progression.

Main Methods:

  • MicroRNA microarray analysis was performed on genetically-modified HCT116 human colon cancer cells cultured as 3D spheroids under hypoxic conditions.
  • Cell growth, stress resistance, and drug sensitivity were assessed in vitro and in vivo for cells engineered to express miR-3148.

Main Results:

  • miR-3148 was identified as a significantly upregulated miRNA in 3D spheroids.
  • HCT116 cells overexpressing miR-3148 exhibited enhanced growth in vivo but not in vitro.
  • miR-3148 conferred resistance to hypoxia in 3D spheroids and increased sensitivity to MAPK/ERK pathway inhibitors.

Conclusions:

  • miR-3148 functions as a novel onco-microRNA, protecting cancer cells from stress, especially in vivo.
  • The mitogen-activated protein kinase (MAPK)/extracellular signal-regulated kinase (ERK) pathway is implicated in miR-3148's protective role.
  • Targeting miR-3148 or the MAPK/ERK pathway may represent a therapeutic strategy for colon cancer.
Keywords:
MicroRNA-3148colon canceronco-miRspheroid

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