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Updated: Feb 4, 2026

Single Particle Electron Microscopy Reconstruction of the Exosome Complex Using the Random Conical Tilt Method
Published on: March 28, 2011
Multicolor single-particle reconstruction of protein complexes
Christian Sieben1,2, Niccolò Banterle3, Kyle M Douglass4
1Laboratory for Experimental Biophysics, Institute of Physics, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland. christian.sieben@epfl.ch.
Abstract:
Single-particle reconstruction (SPR) from electron microscopy (EM) images is widely used in structural biology, but it lacks direct information on protein identity. To address this limitation, we developed a computational and analytical framework that reconstructs and coaligns multiple proteins from 2D super-resolution fluorescence images. To demonstrate our method, we generated multicolor 3D reconstructions of several proteins within the human centriole, which revealed their relative locations, dimensions and orientations.
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