Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Proteomics01:33

Proteomics

9.8K
A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...
9.8K
Frequency-dependent Selection01:21

Frequency-dependent Selection

24.1K
When the fitness of a trait is influenced by how common it is (i.e., its frequency) relative to different traits within a population, this is referred to as frequency-dependent selection. Frequency-dependent selection may occur between species or within a single species. This type of selection can either be positive—with more common phenotypes having higher fitness—or negative, with rarer phenotypes conferring increased fitness.
24.1K
Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches01:23

Types of Biopharmaceutical Studies: Controlled and Non-Controlled Approaches

473
Biopharmaceutical studies constitute a vital field aiming to enhance drug delivery methods and refine therapeutic approaches, drawing upon diverse interdisciplinary knowledge. In research methodologies, the choice between controlled and non-controlled studies significantly influences the study's reliability and accuracy.
Non-controlled studies, commonly employed for initial exploration, lack a control group, rendering them susceptible to biases and external influences. In contrast,...
473
Factors Influencing Attraction I: Proximity01:22

Factors Influencing Attraction I: Proximity

277
Proximity plays a fundamental role in shaping interpersonal attraction by increasing opportunities for interaction and fostering familiarity. Research consistently demonstrates that individuals are more likely to form social bonds with those who are physically closer to them, whether in residential settings, workplaces, or educational institutions. This effect is largely driven by the increased frequency of encounters, which facilitates the development of friendships and romantic...
277
Drug Dependence01:17

Drug Dependence

1.7K
Medications are typically administered to achieve therapeutic effects. Some drugs can modify an individual's mood and perception, frequently resulting in various enjoyable experiences. However, this can result in drug dependency, a condition marked by continuous drug use despite potential negative consequences. Drug dependency primarily falls into two categories: psychological and physical dependence. Psychological dependence occurs when the pleasurable feelings induced by the drug...
1.7K
Contact-dependent Signaling01:19

Contact-dependent Signaling

47.5K
Contact-dependent signaling, as the name suggests, requires that communicating cells be in direct contact with each other. This is achieved either through receptor-ligand interactions or by specialized cytoplasmic channels that allow the flow of small molecules between cells. In animal cells, channels called gap junctions facilitate contact-dependent signaling in certain tissues, whereas, plasmodesmata perform a similar function in plants.
Gap Junctions
In animal cells, gap junctions are formed...
47.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Non-sebaceous lymphadenoma of the salivary gland: case report with immunohistochemical investigation.

Virchows Archiv : an international journal of pathology·2007
Same author

[Enhancement of HSP-MUC1 antitumor activity by type C CpG-ODN BW005].

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology·2007
Same author

Inhibition of severe acute respiratory syndrome-associated coronavirus infection by equine neutralizing antibody in golden Syrian hamsters.

Viral immunology·2007
Same author

Synergistic effect between components of mixtures of cationic amphipaths in transfection of primary endothelial cells.

Molecular pharmaceutics·2007
Same author

The insertion polymorphism in angiotensin-converting enzyme gene associated with the APOE epsilon 4 allele increases the risk of late-onset Alzheimer disease.

Journal of molecular neuroscience : MN·2007
Same author

[Study on the analysis of mixed spectra of benzene homologs with Dolittle multivariate correction method].

Guang pu xue yu guang pu fen xi = Guang pu·2007

Related Experiment Video

Updated: Feb 4, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

3.0K

BioID: A Proximity-Dependent Labeling Approach in Proteomics Study.

Peipei Li1, Yuan Meng1, Li Wang1

  • 1Faculty of Health Sciences, Cancer Center, University of Macau, Macau, China.

Methods in Molecular Biology (Clifton, N.J.)
|October 3, 2018
PubMed
Summary

Understanding protein-protein interactions (PPIs) is crucial for biology. Proximity-dependent labeling (PDL) methods, like BioID, identify interacting proteins by labeling them near a target protein.

Keywords:
BioIDProtein–protein interactionsProximity-dependent labeling

More Related Videos

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

20.5K
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
09:59

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

1.4K

Related Experiment Videos

Last Updated: Feb 4, 2026

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics
11:40

Characterization of Neuronal Lysosome Interactome with Proximity Labeling Proteomics

Published on: June 23, 2022

3.0K
Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment
09:02

Split-BioID — Proteomic Analysis of Context-specific Protein Complexes in Their Native Cellular Environment

Published on: April 20, 2018

20.5K
In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster
09:59

In Vivo Application of TurboID-based Proximity Labeling in Drosophila melanogaster

Published on: June 13, 2025

1.4K

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • Biological functions rely on proteins, often organized into complexes or through protein-protein interactions (PPIs).
  • Identifying these interactions is vital for understanding biological processes.
  • Traditional biochemical methods have limitations in capturing transient or weak interactions.

Purpose of the Study:

  • To introduce and explain the principles of Proximity-Dependent Labeling (PDL) as a method for identifying protein interaction partners.
  • To highlight BioID as a prominent and widely applied PDL technique.

Main Methods:

  • PDL involves fusing a target protein with an enzyme that catalyzes proximity-dependent labeling.
  • A reactive molecule is attached to interacting proteins within a specific distance of the target.
  • Proteins modified by the reactive molecule are identified, typically via mass spectrometry, to reveal interaction partners.

Main Results:

  • PDL methods, exemplified by BioID, enable the discovery of protein interaction candidates.
  • BioID utilizes the BirA biotin ligase enzyme to biotinylate proximal proteins in the presence of biotin.
  • Streptavidin-mediated pull-down followed by mass spectrometry identifies biotinylated proteins as potential interactors.

Conclusions:

  • PDL, particularly BioID, offers a powerful approach to map protein-protein interactions.
  • This technique aids in elucidating the organization of protein complexes and their roles in biological pathways.
  • BioID provides a valuable tool for discovering novel interacting partners and advancing our understanding of molecular mechanisms.