Related Experiment Video
Updated: Feb 4, 2026

Multiplex Cytokine Profiling of Stimulated Mouse Splenocytes Using a Cytometric Bead-based Immunoassay Platform
Published on: November 9, 2017
Glycosylation Profiling of Tumor Marker in Plasma Using Bead-Based Immunoassay
Hongye Wang1, Zheng Cao2, Hu Duan1
1State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (PHOENIX Center, Beijing), Beijing Institute of Lifeomics, Beijing, China.
Abstract:
As one of the most important posttranslational modifications, glycosylation plays critical roles in protein folding, trafficking, cell differentiation, immune recognition, etc. The alteration of glycosylation is closely associated with the pathological processes during and after caner development, and thus holds great value in cancer detection. In this chapter, we describe a protocol on the glycosylation profiling of tumor marker in plasma using bead-based immunoassay with CA125 as a model, including bead coupling, coupling control, glycosylation assay, as well as the plasma screening for breast cancer patients. This protocol can be used to profile the glycosylation of protein markers in clinical plasma or serum samples for different human cancers.
Related Concept Videos
Protein Glycosylation
Glycosylation occurs in...
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
Composition of Blood Plasma
Enlargement of the Plasma Membrane
Plasma Membrane in Bacteria and Archaea
Taping Over Different Ground Profiles

