Related Experiment Video
Updated: Feb 4, 2026
12:12
On the Preparation and Testing of Fuel Cell Catalysts Using the Thin Film Rotating Disk Electrode Method
Published on: March 16, 2018
22.9K
[The Deveroppment of Screening Methods Using the Disk Diffusion Method for Carbapenemase-producing
Summary
Rapidly identify carbapenemase-producing Enterobacteriaceae (CPE) using faropenem disks. This method offers high sensitivity and specificity for effective screening of these critical antimicrobial-resistant infections.
Area of Science:
- Microbiology
- Infectious Diseases
- Clinical Diagnostics
Background:
- Carbapenem-resistant Enterobacteriaceae (CRE), especially carbapenemase-producing Enterobacteriaceae (CPE), pose a growing global health threat.
- Accurate and timely detection of CPE is crucial for guiding antimicrobial therapy and implementing hospital infection control measures.
Purpose of the Study:
- To evaluate the efficacy of a carbapenem susceptibility disk, specifically faropenem (FRPM), for the rapid screening of carbapenemase-producing Enterobacteriaceae (CPE).
Main Methods:
- Carbapenem susceptibility testing was performed using various carbapenem disks, including faropenem (5 μg), imipenem (10 μg), and biapenem (10 μg).
- Minimal inhibition zone diameters were measured for carbapenemase-producing strains.
- Receiver Operating Characteristic (ROC) analysis was used to determine optimal cutoff values for differentiating carbapenemase producers.
Main Results:
- Carbapenemase producers exhibited minimal inhibition zones with faropenem (5 μg) disks (mean: 6.9 mm).
- Faropenem disks demonstrated the highest sensitivity (100%) for CPE screening.
- While specificities varied, faropenem showed acceptable performance (85.25%) compared to other tested agents.
Conclusions:
- Drug susceptibility testing with faropenem (5 μg) disks provides a simple, sensitive, and accurate method for screening carbapenemase-producing Enterobacteriaceae.
- This approach can aid in the early identification of CPE, facilitating prompt clinical and infection control interventions.
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