Related Experiment Video
Updated: Feb 4, 2026

Matrix-assisted Laser Desorption/Ionization Time of Flight MALDI-TOF Mass Spectrometric Analysis of Intact Proteins Larger than 100 kDa
Published on: September 9, 2013
Matrix-Incorporated Polydopamine Layer as a Simple, Efficient, and Universal Coating for Laser Desorption/Ionization
Young-Kwan Kim1, Ryan Landis2, Richard W Vachet2
1Carbon Composite Materials Research Center , Korea Institute of Science and Technology , 92 Chudong-ro , Bongdong-eup, Wanju-gun , Jeollabuk-do 55424 , South Korea.
Abstract:
Self-oxidative copolymerization of dopamine with α-cyano-4-hydroxycinnamic acid (CHCA) provides an efficient and multifunctional platform for laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF-MS) analysis. The polydopamine coating layer embedded with the CHCA matrix can be readily formed on nanomaterials and solid substrates without additional surface treatments to generate an efficient LDI-TOF-MS platform for the analysis of small molecules as well as synthetic polymers. This coating can be further functionalized with specific ligands for target enrichment from complex biological media, providing analyte capture for subsequent LDI-TOF-MS analysis.
More Related Videos
09:38Dithranol as a Matrix for Matrix Assisted Laser Desorption/Ionization Imaging on a Fourier Transform Ion Cyclotron Resonance Mass Spectrometer
Published on: November 26, 2013
12:19Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis
Published on: April 22, 2022
Related Concept Videos
Matrix-Assisted Laser Desorption Ionization (MALDI)
Chemical Ionization (CI) Mass Spectrometry
Electrospray Ionization (ESI) Mass Spectrometry
ESI utilizes electrical energy to transfer ions from the liquid phase of the sample into the...
Ionization Energy
Mass Spectrometry: Complex Analysis
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...
The Extracellular Matrix