Related Experiment Video

Updated: Feb 4, 2026

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation
08:07

Personalized Peptide Arrays for Detection of HLA Alloantibodies in Organ Transplantation

Published on: September 6, 2017

10.6K

Sequence-based HLA-A, B, C, DP, DQ, and DR typing of 496 adults from San Diego, California, USA

Eugene Moore1, Alba Grifoni1, Daniela Weiskopf1

  • 1Division of Vaccine Discovery, La Jolla Institute for Allergy & Immunology, La Jolla, CA 92037, USA.

Human Immunology
|October 3, 2018
PubMed

Insights

This study characterized Human Leukocyte Antigen (HLA) allele frequencies in 496 healthy San Diego adults. High frequencies of specific HLA class I and class II alleles were identified, aiding T cell response research.

Area of Science:

  • Immunogenetics
  • Population genetics

Background:

  • The Human Leukocyte Antigen (HLA) system plays a crucial role in immune responses.
  • Understanding HLA allele frequencies in diverse populations is essential for immunological studies, including T cell responses to allergens.

Purpose of the Study:

  • To determine the allele frequencies of specific HLA loci (HLA-A, -B, -C, -DPB1, -DQA1, -DQB1, and -DRB1) in a healthy adult cohort from San Diego, California.
  • To provide foundational data for research investigating T cell responses to common allergens.

Main Methods:

  • DNA sequencing was employed for high-resolution typing of HLA loci.
  • Analysis included 496 healthy adult donors from San Diego, California.
  • Deviations from Hardy-Weinberg proportions were assessed for each locus.

Main Results:

  • Allele frequencies were characterized across seven HLA loci.
  • Deviations from Hardy-Weinberg equilibrium were observed at all loci except HLA-A and HLA-C.
  • Several alleles, including DPB1∗02:01, DPB1∗04:01, DQA1∗01:02, DQA1∗05:01, DQB1∗03:01 (class II), and A∗02:01 (class I), were present in over 15% of individuals.
  • Genotype data will be accessible via the Allele Frequencies Net Database (AFND 3562).

Conclusions:

  • The study provides valuable HLA allele frequency data for a Southern California population.
  • These findings support research on T cell-mediated immune responses, particularly in the context of allergen sensitization.
  • The characterized frequencies contribute to a better understanding of HLA polymorphism and its implications in health and disease.

Keywords:
AllergyHLA allelesHLA typingSan Diego, California

More Related Videos

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.4K
Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis06:42

Cerebrovascular Casting of the Adult Mouse for 3D Imaging and Morphological Analysis

Published on: November 30, 2011

22.3K

Related Concept Videos

Adult Stem Cells01:33

Adult Stem Cells

Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.9K
Types of RNA01:23

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...
72.9K
Cis-regulatory Sequences02:02

Cis-regulatory Sequences

Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.8K
Classification of Titrimetric Analysis Based on Reaction Types01:01

Classification of Titrimetric Analysis Based on Reaction Types

Titrimetric analysis in solution chemistry involves measuring the volume of solutions and is often called volumetric analysis. The standard solution of known concentration in the burette is called the titrant, whereas the solution of unknown concentration in the flask is called the analyte, or titrand. Titrimetric analyses can be classified into four types based on the reactions between the titrant and analyte.
Titrations between an acid and a base lead to neutralization reactions that form...
1.8K
Sequences01:29

Sequences

Sequences are fundamental mathematical objects consisting of ordered lists of numbers that follow a specific rule or pattern. Sequences are critical in various mathematical concepts, including calculus, series, and number theory. They can model real-world phenomena such as population growth, financial investments, and physical processes like the diminishing height of a bouncing ball.Each number in a sequence is referred to as a term. Typically, the terms are denoted as a1, a2, a3,…, where...
278
Sanger Sequencing01:57

Sanger Sequencing

DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
774.7K