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Repressed Memory01:16

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Repressed memories are a psychological phenomenon where memories of traumatic events are unconsciously blocked from a person's awareness. This process occurs as a defense mechanism, protecting the mind from the emotional impact of distressing or painful experiences. For example, a person who has experienced childhood trauma may grow up with no conscious recollection of the event. In such cases, the memories are thought to be buried deep within the subconscious, inaccessible to the conscious...
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Related Experiment Video

Updated: Feb 4, 2026

Author Spotlight: A Computational Pipeline for Analyzing Chimeric Noncoding RNA-Target RNA Interactions in High-Throughput Sequencing Data
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Long noncoding RNA SYISL regulates myogenesis by interacting with polycomb repressive complex 2.

Jian Jun Jin1,2,3, Wei Lv1,2,3, Pan Xia1,2,3

  • 1Key Laboratory of Swine Genetics and Breeding of the Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, 430070 Wuhan, Hubei, People's Republic of China.

Proceedings of the National Academy of Sciences of the United States of America
|October 4, 2018
PubMed
Summary
This summary is machine-generated.

SYISL, a muscle-expressed long noncoding RNA, promotes muscle growth by recruiting EZH2/PRC2 to silence differentiation genes. SYISL knockout increases muscle mass, revealing its role as a key repressor in myogenesis.

Keywords:
H3K27 trimethylationPRC2SYISLlncRNAmyogenesis

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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Muscle Development

Background:

  • Long noncoding RNAs (lncRNAs) are increasingly recognized for their roles in cellular processes, yet their functions in muscle remain largely unknown.
  • Understanding lncRNA expression and function during myogenesis is crucial for deciphering muscle regulatory networks.

Purpose of the Study:

  • To systematically characterize lncRNA expression during C2C12 myoblast differentiation.
  • To identify and functionally characterize novel lncRNAs involved in muscle development.
  • To elucidate the molecular mechanisms underlying the function of identified lncRNAs in myogenesis.

Main Methods:

  • RNA sequencing to profile lncRNA expression during C2C12 myoblast differentiation.
  • Functional characterization of the lncRNA SYISL using cell-based assays (proliferation, fusion, differentiation) and in vivo mouse models (SYISL knockout).
  • Chromatin immunoprecipitation followed by sequencing (ChIP-seq) and Western blotting to investigate the interaction of SYISL with EZH2/PRC2 and its effect on target gene epigenetic modifications.

Main Results:

  • Identification of SYISL, an intronic lncRNA highly expressed in muscle, during C2C12 myoblast differentiation.
  • SYISL promotes myoblast proliferation and fusion while inhibiting myogenic differentiation.
  • SYISL knockout in mice leads to increased muscle fiber density and overall muscle mass.
  • Mechanistically, SYISL recruits EZH2/PRC2 to silence key muscle-specific genes (p21, MyoG, MCK, Myh4) via H3K27 trimethylation.

Conclusions:

  • SYISL acts as a critical repressor of muscle development by mediating EZH2/PRC2-dependent epigenetic silencing.
  • SYISL plays a significant role in regulating myogenesis and muscle mass.
  • Targeting SYISL may offer therapeutic strategies for muscle-related disorders.